Organic Spherical Nucleic Acids for the Transport of a NIR-II-Emitting Dye Across the Blood-Brain Barrier
Organic Spherical Nucleic Acids for the Transport of a NIR-II-Emitting Dye Across the Blood-Brain Barrier
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用于跨血脑屏障运输 NIR-II 发射染料的有机球形核酸
DOI：
10.1002/anie.202002312
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发表时间：
2020-04-02
影响因子：
16.6
通讯作者：
Tian, Leilei
中科院分区：
文献类型：
--
作者：
Xiao, Fan;Lin, Lin;Tian, Leilei
DNA纳米技术在生物医学领域发挥着越来越重要的作用，但其在有机纳米材料设计中的应用还没有得到充分的探索。在这里，我们报告了利用DNA纳米技术将发射NIR-II的纳米荧光团运送到血脑屏障(BBB)，促进脑肿瘤的非侵入性成像。具体地，DNA嵌段共聚物PS-b-DNA是通过固相点击反应合成的。我们证明了它的自组装结构表现出了特殊的团簇效应，其中最显著的是BBB交叉效应。因此，我们利用PS-b-DNA作为两亲性基质来制备NIR-II纳米荧光团，并将其应用于活体生物成像。因此，定位于胶质母细胞瘤的基于DNA的纳米荧光团的NIR-II荧光信号比基于聚乙二醇基的对应物的NIR-II荧光信号高3.8倍。显着提高的成像分辨率将大大有助于脑肿瘤的进一步诊断和治疗。
DNA nanotechnology plays an increasingly important role in the biomedical field; however, its application in the design of organic nanomaterials is underexplored. Herein, we report the use of DNA nanotechnology to transport a NIR-II-emitting nanofluorophore across the blood-brain barrier (BBB), facilitating non-invasive imaging of brain tumors. Specifically, the DNA block copolymer, PS-b-DNA, is synthesized through a solid-phase click reaction. We demonstrate that its self-assembled structure shows exceptional cluster effects, among which BBB-crossing is the most notable. Therefore, PS-b-DNA is utilized as an amphiphilic matrix to fabricate a NIR-II nanofluorephore, which is applied in in vivo bioimaging. Accordingly, the NIR-II fluorescence signal of the DNA-based nanofluorophore localized at a glioblastoma is 3.8-fold higher than the NIR-II fluorescence signal of the PEG-based counterpart. The notably increased imaging resolution will significantly benefit the further diagnosis and therapy of brain tumors.