Puerarin blocks the signaling transmission mediated by P2X3 in SG and DRG to relieve myocardial ischemic damage
Puerarin blocks the signaling transmission mediated by P2X3 in SG and DRG to relieve myocardial ischemic damage
复制标题
葛根素阻断SG和DRG中P2X3介导的信号传导减轻心肌缺血损伤
DOI：
10.1016/j.brainresbull.2014.01.001
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发表时间：
2014-02-01
影响因子：
3.8
通讯作者：
Liang, Shangdong
中科院分区：
文献类型：
--
作者：
Liu, Shuangmei;Zhang, Chunping;Liang, Shangdong
星状神经节（SG）和颈背根神经节（DRG）神经元中的P2X(3)受体参与心肌缺血损伤诱导的交感兴奋反射。葛根素是一种从中药葛根中提取的重要有效成分，已被广泛用于治疗心肌和脑缺血。本研究旨在观察葛根素对心肌缺血损伤后SG和DRG中P2X3受体介导的信号传递的影响。结果表明，心肌缺血损伤后，大鼠收缩压和心率升高，SG和DRG中P2X3 mRNA和蛋白表达水平上调。葛根素降低心肌缺血后收缩压和心率，减轻疼痛，降低SG和DRG中P2X3 mRNA和蛋白的上调表达。葛根素抑制心肌缺血后DRG神经元atp激活电流的上调。由此可见，葛根素可通过阻断P2X3信号传递，进而抑制交感兴奋反射加重，从而减轻心肌缺血损伤。(C) 2014爱思唯尔公司版权所有。
P2X(3) receptors in stellate ganglia (SG) and cervical dorsal root ganglia (DRG) neurons are involved in sympathoexcitatory reflex induced by myocardial ischemic damage. Puerarin, a major active ingredient extracted from the traditional Chinese plant medicine Ge-gen, has been widely used in treatment of myocardial and cerebral ischemia. The present study is aimed to observe the effects of puerarin on the signaling transmission mediated by P2X3 receptor in SG and DRG after myocardial ischemic damage. Our results showed that systolic blood pressure and heart rate increased, and the expression levels of P2X3 mRNA and protein in SG and DRG were up-regulated after myocardial ischemic damage. Puerarin reduced systolic blood pressure and heart rate, relieved pain and decreased up-regulated expression of P2X3 mRNA and protein in SG and DRG after myocardial ischemia. Puerarin inhibited the up-regulated ATP-activated currents in DRG neurons after myocardial ischemia. Thus, puerarin can relieve myocardial ischemic damage through blocking the P2X3 signaling transmission and then depressed the aggravated sympathoexcitatory reflex. (C) 2014 Elsevier Inc. All rights reserved.