C-C chemokines released by lipopolysaccharide (LPS)-stimulated human macrophages suppress HIV-1 infection in both macrophages and T cells.
C-C chemokines released by lipopolysaccharide (LPS)-stimulated human macrophages suppress HIV-1 infection in both macrophages and T cells.
复制标题
通过脂多糖（LPS）刺激的人类巨噬细胞释放的C-C趋化因子抑制了巨噬细胞和T细胞中的HIV-1感染。
DOI：
10.1084/jem.185.5.805
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发表时间：
1997-03-03
影响因子：
15.3
通讯作者：
Vercelli, D
中科院分区：
文献类型：
--
作者：
Verani, A;Scarlatti, G;Comar, M;Tresoldi, E;Polo, S;Giacca, M;Lusso, P;Siccardi, AG;Vercelli, D
已知体外感染的单核细胞源性巨噬细胞（MDM）中的人类免疫缺陷病毒-1 （HIV-1）表达可被脂多糖（LPS）抑制。然而，其机制尚不完全清楚。我们在这里表明，HIV-1的抑制是由生理上显著浓度的LPS刺激的MDM释放的可溶性因子介导的。lps条件下的MDM上清液抑制了MDM和T细胞中的HIV-1复制。C-C趋化因子（RANTES、MIP-1α和MIP-1β）的缺失抵消了lps条件下的上清液在MDM中抑制HIV-1复制的能力。重组C-C趋化因子组合阻断HIV-1感染的效果与LPS相同。在这里，我们报道了C-C趋化因子对原代巨噬细胞中HIV复制的抑制作用。我们的研究结果提高了单核细胞可能在HIV感染中发挥双重作用的可能性：作为病毒的储存库，它们可能通过释放抑制HIV复制的因子来抑制感染，不仅在单核细胞中，而且在T淋巴细胞中。
Human immunodeficiency virus-1 (HIV-1) expression in monocyte-derived macrophages (MDM) infected in vitro is known to be inhibited by lipopolysaccharide (LPS). However, the mechanisms are incompletely understood. We show here that HIV-1 suppression is mediated by soluble factors released by MDM stimulated with physiologically significant concentrations of LPS. LPS-conditioned supernatants from MDM inhibited HIV-1 replication in both MDM and T cells. Depletion of C–C chemokines (RANTES, MIP-1α, and MIP-1β) neutralized the ability of LPS-conditioned supernatants to inhibit HIV-1 replication in MDM. A combination of recombinant C–C chemokines blocked HIV-1 infection as effectively as LPS. Here, we report an inhibitory effect of C–C chemokines on HIV replication in primary macrophages. Our results raise the possibility that monocytes may play a dual role in HIV infection: while representing a reservoir for the virus, they may contribute to the containment of the infection by releasing factors that suppress HIV replication not only in monocytes but also in T lymphocytes.
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影响因子：
56.9
作者：
Feng, Y;Broder, CC;Berger, EA
通讯作者：
Berger, EA
影响因子：
56.9
作者：
COCCHI, F;DEVICO, AL;LUSSO, P
通讯作者：
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DOI：
10.1073/pnas.86.7.2365
发表时间：
1989-04-01
影响因子：
11.1
作者：
FOLKS, TM;CLOUSE, KA;FAUCI, AS
通讯作者：
FAUCI, AS
影响因子：
15.9
作者：
BERNSTEIN, MS;TONGSTARKSEN, SE;LOCKSLEY, RM
通讯作者：
LOCKSLEY, RM
影响因子：
1.5
作者：
Comar, M;Marzio, G;Giacca, M
通讯作者：
Giacca, M