Visceral Leishmania tropica infection of BALB/c mice: cellular analysis of in vitro unresponsiveness to sheep erythrocytes
Visceral Leishmania tropica infection of BALB/c mice: cellular analysis of in vitro unresponsiveness to sheep erythrocytes
复制标题
BALB/c 小鼠内脏热带利什曼原虫感染：体外对绵羊红细胞无反应的细胞分析
DOI：
10.1128/iai.37.3.895-902.1982
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发表时间：
1982
影响因子：
3.1
通讯作者：
L. Chedid
中科院分区：
文献类型：
--
作者：
C. Leclerc;F. Modabber;E. Dériaud;J. Djoko;L. Chedid
在小鼠中，感染热带利什曼原虫最初产生了非特异性增强的免疫反应，绵羊红细胞在体外和体内测量。随后，敏感小鼠（BALB/c）的脾细胞反应绵羊红细胞和T-和B-细胞有丝分裂原在体外显着下降，而那些抗性品系（C57 BL/6）恢复正常。对感染动物脾细胞的分析表明，巨噬细胞（利什曼原虫的靶细胞）没有缺陷。然而，T-和B-细胞耗尽的感染动物的脾细胞群体缺乏在其相应的B-和T-细胞耗尽的正常脾细胞群体存在下的应答能力。还观察到，加入不同数量的利什曼原虫生物体不会改变体外正常脾细胞的反应。不同比例的感染动物和正常动物脾脏细胞共培养的结果排除了强活性免疫抑制的可能性。体外应答的降低归因于感染小鼠脾脏中免疫活性细胞的耗竭，其大量由空细胞占据。
In mice, infection with Leishmania tropica initially produced a nonspecific enhancement of the immune response to sheep erythrocytes as measured both in vitro and in vivo. Subsequently, the spleen cell responses of susceptible mice (BALB/c) to sheep erythrocytes and T- and B-cell mitogens in vitro decreased dramatically, whereas those of the resistant strain (C57BL/6) returned to normal. Analysis of the spleen cells of infected animals revealed that macrophages (the target cells of Leishmania) were not defective. However, both T- and B-cell-depleted splenocyte populations of infected animals lacked the ability to respond in the presence of their corresponding B- and T-cell-depleted populations of normal spleen cells. It was also observed that the addition of various numbers of Leishmania organisms did not alter the response of normal spleen cells in vitro. The results of cocultures of various ratios of cells from the spleen of infected and normal animals ruled out the possibility of a strong active immunosuppression. The decrease of in vitro response is attributed to the depletion of immunocompetent cells in the spleen of infected mice, which is heavily populated by null cells.