Effect of high temperature during larval and juvenile stages on masculinization of common carp (Cyprinus carpio, L)
Effect of high temperature during larval and juvenile stages on masculinization of common carp (Cyprinus carpio, L)
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DOI：
10.1016/j.aquaculture.2020.735803
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发表时间：
2021-01
期刊：
影响因子：
4.5
通讯作者：
C. Biswas;S. Chakraborty;S. Munilkumar;P. Gireesh-Babu;P. Sawant;N. K. Chadha;G. Krishna;S. Dasgupta
中科院分区：
文献类型：
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作者：
C. Biswas;S. Chakraborty;S. Munilkumar;P. Gireesh-Babu;P. Sawant;N. K. Chadha;G. Krishna;S. Dasgupta
温度可以通过遗传性别决定系统（GSD）影响雌雄异株鱼类的性别分化。高温对性的确切影响在广温性鲤鱼（Cyprinus carpio）中仍然未知。本研究旨在确定鲤鱼性别分化的临界温度敏感期及温度对其性别分化的影响。carpio。结果表明，该菌的致死最高温度（LTmax）的上下限均为0. Carpiojuvenile（42 dph）在本研究条件下分别为37.2和39.7 °C。幼虫的半数致死时间（LT 50）的置信限为38.87 °C。为了确定性别分化的温度敏感性，将幼虫暴露于高温（36 °C）持续7天，从孵化开始直到69 dph。雄性表型性别分化的临界温度敏感期为7 ~ 13 dph。性别比例和性别分化的温度敏感性表明，在暴露于36 °C的10个全同胞家系中，性别比例向雄性表型倾斜的家族间变异范围为69%至100%。幼鱼暴露于高温（34 °C和36 °C）显著改变了性比，即使在性别分化的过渡期间也产生了63%至82%的雄性表型。本研究表明，鲤鱼性别分化受环境过程的严重影响。此外，它还探讨了一种温度诱导的方法，使鲤鱼的性比向雄性表型倾斜，而不考虑性腺的形态分化。
Temperature can influence sex differentiation in gonochoristic fish with a genetic sex-determination system (GSD). The precise effect of high temperature on sex remains unknown in a eurythermal carp (Cyprinus carpio). The present study aimed to identify the critical thermosensitive period and the effect of temperature on sex differentiation in common carp,C. carpio. Our results demonstrated the lower and upper limits of the lethal maximum temperature (LTmax) ofC. carpiojuveniles (42 dph) were 37.2 and 39.7 °C, respectively in the present study condition. The confidence limit of the median lethal time (LT50) of the juveniles was 38.87 °C. To determine the thermosensitivity of sex differentiation, the larvae were exposed to a high temperature (36 °C) for 7-day durations, starting from hatching until 69 dph. The period of critical thermosensitivity for sex differentiation towards the male phenotype was 7 to 13 dph. The sex proportions and thermosensitivity of sex differentiation illustrated high inter-familial variability of sex ratio skewed towards male phenotype ranged from 69 to 100% in the 10 full-sib families exposed to 36 °C. Exposure of juveniles to the high temperatures (34 °C and 36 °C) significantly altered the sex ratios and produced 63 to 82 % male phenotype even during the transition of sex differentiation. This study demonstrates that sex differentiation in common carp is seriously influenced by the environmental process. Also, it explores a temperature-induced method of skewing sex ratio towards the male phenotype irrespective of morphological gonad differentiation in common carp.