Emodin induces apoptosis of human breast cancer cells by modulating the expression of apoptosis-related genes
Emodin induces apoptosis of human breast cancer cells by modulating the expression of apoptosis-related genes
复制标题
大黄素通过调节凋亡相关基因的表达诱导人乳腺癌细胞凋亡
DOI：
10.3892/ol.2015.3646
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发表时间：
2015-11-01
期刊：
影响因子：
2.9
通讯作者：
Zheng, Xinyu
中科院分区：
文献类型：
--
作者：
Zu, Cong;Zhang, Mingdi;Zheng, Xinyu
本研究旨在探讨大黄素对人乳腺癌细胞Bcap-37和ZR-75-30增殖的影响。大黄素处理后的细胞活力通过3-（4，5-二甲基噻唑-2-基）-2，5-二苯基四唑溴化物（MTT）测定来评估。流式细胞仪Annexin V-异硫氰酸荧光素染色和碘化丙啶染色检测大黄素对细胞凋亡的影响。定量聚合酶链反应和蛋白质印迹分析分别用于确定凋亡基因和蛋白质表达的变化。采用Matrigel侵袭实验检测大黄素对乳腺癌细胞侵袭能力的影响。大黄素对乳腺癌细胞Bcap-37和ZR-75-30的生长有明显的抑制作用，并呈时间和剂量依赖性诱导细胞凋亡。大黄素降低Bcl-2的水平，增加切割的caspase-3、PARP、p53和Bax的水平。这些结果表明，大黄素诱导人乳腺癌细胞的生长抑制和凋亡。大黄素可能是一种潜在的治疗乳腺癌的药物。
The aim of this study was to investigate the effects of emodin on the proliferation of human breast cancer cells Bcap-37 and ZR-75-30. Cell viability following emodin treatment was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The effects of emodin on apoptosis were determined by flow cytometry using Annexin V-fluorescein isothiocyanate and propidium iodide staining. Quantitative polymerase chain reaction and western blot analysis were used to determine changes in the expression of apoptotic genes and protein, respectively. The effect of emodin on the invasiveness of breast cancer cells was evaluated by Matrigel invasion assay. Treatment of breast cancer cells Bcap-37 and ZR-75-30 with emodin was observed to inhibit the growth and induced apoptosis in a time-and dose-dependent manner. Emodin reduced the level of Bcl-2 and increased levels of cleaved caspase-3, PARP, p53 and Bax. These findings indicate that emodin induces growth inhibition and apoptosis in human breast cancer cells. Emodin may be a potential therapeutic agent for the treatment of breast cancer.