Simultaneous determination of blood glucose and isoleucine levels in rats after chronic alcohol exposure by microwave-assisted derivatization and isotope dilution gas chromatography/mass spectrometry.
Simultaneous determination of blood glucose and isoleucine levels in rats after chronic alcohol exposure by microwave-assisted derivatization and isotope dilution gas chromatography/mass spectrometry.
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DOI：
10.1002/rcm.3350
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发表时间：
2008-01
期刊：
影响因子：
--
通讯作者：
Ruyi Xue;Si Zhang;Chunhui Deng;Ling Dong;Tao-Tao Liu-Tao;Ji-yao Wang;Hongyi Wu;J. Gu;Xizhong Shen
中科院分区：
文献类型：
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作者：
Ruyi Xue;Si Zhang;Chunhui Deng;Ling Dong;Tao-Tao Liu-Tao;Ji-yao Wang;Hongyi Wu;J. Gu;Xizhong Shen
血糖和异亮氨酸是慢性酒精暴露的两个生物标志物。同时测定血糖和异亮氨酸水平有助于阐明酒精对葡萄糖和氨基酸代谢的影响。最准确的血清葡萄糖检测方法是同位素稀释气相色谱/质谱法（ID GC/MS）。本研究建立了一种快速、简便、灵敏的微波辅助衍生化（MAD）和ID GC/MS定量分析慢性酒精暴露大鼠血清葡萄糖和异亮氨酸的方法。用GC/MS分析了三甲基硅衍生物。这种技术被用来证明吡咯喹啉醌（PQQ），一种参与某些糖或醇代谢的醌蛋白中的非共价结合辅基，可以逆转酒精暴露诱导的血糖升高。另一方面，它不影响异亮氨酸的代谢，其水平沿着血清葡萄糖升高。研究表明，MAD和ID GC/MS相结合是一种准确、快速、简单、灵敏的血清样品中葡萄糖和异亮氨酸定量方法。
Blood glucose and isoleucine are two biomarkers of chronic alcohol exposure. Simultaneous determination of blood glucose and isoleucine levels helps to illuminate the influence of alcohol on the metabolism of glucose and amino acids. The most accurate method for the detection of serum glucose is isotope dilution gas chromatography/mass spectrometry (ID GC/MS). In this study, a rapid, simple and sensitive technique was developed for the quantitative analysis of glucose and isoleucine in rats after chronic alcohol exposure by microwave-assisted derivatization (MAD) and ID GC/MS. Serum glucose and isoleucine were rapidly derivatized by N-methyl-N-trimethylsilyltrifluoroacetamide (MSTFA) with microwave irradiation, and the trimethylsilyl derivatives were analyzed by GC/MS. This technique was used to demonstrate that pyrroloquinoline quinone (PQQ), a non-covalently bound prosthetic group in some quinoproteins involved in the metabolism of some sugar or alcohol, could reverse alcohol exposure induced glucose elevation. On the other hand, it did not affect the metabolism of isoleucine whose level was elevated along with serum glucose. The combination of MAD and ID GC/MS has been shown to be an accurate, rapid, simple and sensitive method for the quantification of glucose and isoleucine in serum samples.