DERIVATION OF A DNA CLONE CORRESPONDING TO THE VIRAL AGENT OF SHEEP-ASSOCIATED MALIGNANT CATARRHAL FEVER
DERIVATION OF A DNA CLONE CORRESPONDING TO THE VIRAL AGENT OF SHEEP-ASSOCIATED MALIGNANT CATARRHAL FEVER
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DOI：
10.1016/0034-5288(91)90051-o
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发表时间：
1991-01-01
影响因子：
2.4
通讯作者：
REID, HW
中科院分区：
文献类型：
--
作者：
BRIDGEN, A;REID, HW
恶性卡他热是反刍动物致命的淋巴增殖性和退行性疾病。 一种病原体是伽马疱疹病毒阿尔塞拉芬疱疹病毒 1 (AHV-1)，它不会在其天然宿主角马（Connochaetes 物种）中产生疾病。 流行病学证据表明绵羊是类似病毒的携带者。 然而，从绵羊或患有绵羊相关恶性卡他热（SA-MCF）的动物中培养这种病毒的尝试失败了。 已使用 SA-MCF 从牛、鹿和兔中繁殖类淋巴母细胞。 尽管这些细胞没有显示出病毒颗粒或抗原的证据，但杂交实验现在表明它们含有与 AHV-1 的 DNA 序列同源的 DNA 序列。 从这些类淋巴母细胞系之一构建了基因组文库，并鉴定了与克隆的 AHV-1 DNA 杂交的克隆。 作者认为，该克隆包含SA-MCF病毒基因组的一部分，并且SA-MCF病毒和AHV-1是密切相关的伽玛疱疹病毒。
Malignant catarrhal fever is a fatal lymphoproliferative and degenerative disease of ruminants. One causative agent is the gammaherpesvirus alcelaphine herpesvirus 1 (AHV-1), which produces no disease in its natural host, the wildebeest (Connochaetes species). Epidemiological evidence implicates sheep as the carrier of a similar virus. However, attempts to culture this virus from sheep or from animals affected with sheep-associated malignant catarrhal fever (SA-MCF) have failed. Lymphoblastoid cells have been propagated from cattle, deer and rabbits with SA-MCF. Although these cells show no evidence of viral particles or antigens, hybridisation experiments now show that they contain DNA sequences homologous to those of AHV-1. A genomic library was constructed from one of these lymphoblastoid cell lines and a clone identified which hybridised to cloned AHV-1 DNA. The authors believe that this clone contains part of the SA-MCF viral genome, and that the SA-MCF virus and AHV-1 are closely related gammaherpesviruses.