Remote Limb Ischemic Conditioning during Cerebral Ischemia Reduces Infarct Size through Enhanced Collateral Circulation in Murine Focal Cerebral Ischemia
Remote Limb Ischemic Conditioning during Cerebral Ischemia Reduces Infarct Size through Enhanced Collateral Circulation in Murine Focal Cerebral Ischemia
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DOI：
10.1016/j.jstrokecerebrovasdis.2017.09.068
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发表时间：
2018-04-01
影响因子：
2.5
通讯作者：
Shimizu, Satoru
中科院分区：
文献类型：
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作者：
Kitagawa, Kazuo;Saitoh, Moeko;Shimizu, Satoru
背景：远端缺血调节（RIC）对局灶性脑缺血具有保护作用。条件作用分为前条件作用、后条件作用和后条件作用。然而，RIC的机制尚不清楚。目的：本研究旨在确定最有效的RIC亚型。我们还检查了RIC的侧支循环的受累情况。方法：在监测脑血流量（CBF）的情况下，用尼龙缝合线对成年C57BL/6小鼠进行短暂性大脑中动脉闭塞术（MCAO）。50只小鼠分为5组：MCAO对照组、延迟预RIC组（MCAO前24小时）、早期预RIC组（MCAO前5分钟）、预RIC组（MCAO期间）和后RIC组（MCAO后5分钟）。在其他大脑中动脉（MCA）对照组和per-RIC组，用乳胶复合物灌注观察侧枝循环。结果：MCAO后各组CBF均降低80%。MCAO结束时，每ric组CBF的相对增加明显大于MCA对照组，而每ric组的梗死面积明显小于其他组。per-RIC组小脑膜吻合口直径明显大于对照组。结论：在4例RIC手术中，只有每RIC组表现出明显的脑保护作用。侧枝循环的增强可能在per-RIC的保护作用中起作用。
Background: Remote ischemic conditioning (RIC) induces protection in focal cerebral ischemia. The conditioning is divided into pre-, per-, and postconditioning. However, the mechanisms of RIC remain unknown. Objectives: This study aimed to determine the most effective subtype of RIC. We also examined involvement of collateral circulation on RIC. Methods: Transient middle cerebral artery occlusion (MCAO) was performed with nylon sutures in adult C57BL/6 mice under the monitoring of cerebral blood flow (CBF). Fifty mice were divided into 5 groups: MCAO control group, delayed pre-RIC group (RIC 24 hours before MCAO), early pre-RIC group (RIC 5 minutes before MCAO), per-RIC group (RIC during MCAO), and post-RIC group (RIC 5 minutes after MCAO). In other middle cerebral artery (MCA) control and per-RIC groups, collateral circulation was visualized with latex compound perfusion. Results: After MCAO, CBF was reduced by 80% in all groups. At the end of MCAO, relative increase in CBF in per-RIC group was significantly greater than that in MCA control, whereas the infarct volume in per-RIC group was significantly smaller than that in other groups. The diameter of leptomeningeal anastomosis was larger in the per-RIC group than that in the control group. Conclusions: Among the 4 RIC procedures, only the per-RIC group showed clear brain protection. Enhancement of collateral circulation could play a role in the protective effect of per-RIC.