The complete genome sequence of a novel mycovirus from Alternaria longipes strain HN28
The complete genome sequence of a novel mycovirus from Alternaria longipes strain HN28
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长足链格孢 HN28 株新型真菌病毒的完整基因组序列
DOI：
10.1007/s00705-014-2218-9
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发表时间：
2015-02-01
影响因子：
2.7
通讯作者：
Guo, Lihua
中科院分区：
文献类型：
--
作者：
Lin, Yanhong;Zhang, Hailong;Guo, Lihua
测定并分析了一种新型双链RNA(DsRNA)真菌病毒--链格孢霉dsRNA病毒1(AlRV1)的全序列。AlRV1-HN28包含一个长度为3415个碱基对的dsRNA基因组片段(不包括3‘聚(A)尾巴)，并被预测包含两个不连续的开放阅读框架(ORF、ORF A和ORF B)。5‘-近端ORF A(1182个核苷酸)可能编码一个394个氨基酸(AA)的蛋白质，预测的相对分子质量为43 kDa；该蛋白质与NCBI蛋白质数据库中的任何其他序列没有显著的相似性。3‘端开放阅读框B(1737nt)编码579aa的蛋白质，预测分子质量为65 kDa，该蛋白质序列与其他真菌病毒依赖RNA聚合酶的保守结构域有相似之处。系统发育分析表明，AlRV1-HN28与其他4种未分类病毒有较近的亲缘关系，提示AlRV1-HN28可能属于一个新的dsRNA真菌病毒家族。这是首次报道感染链格孢菌的真菌病毒的全长核苷酸序列。
The complete nucleotide sequence of Alternaria longipes dsRNA virus 1 (AlRV1), a novel double-stranded RNA (dsRNA) mycovirus, was determined and analyzed. AlRV1-HN28 contains a single dsRNA genome segment 3415 base pairs in length (excluding the 3' poly(A) tail) and was predicted to contain two discontiguous open reading frames (ORFs, ORF A and ORF B). The 5'-proximal ORF A (1182 nt) potentially encodes a protein of 394 amino acids (aa) with a predicted molecular mass of 43 kDa; this protein showed no significant similarities to any other sequences in any of the NCBI protein databases. The 3'-proximal ORF B (1737 nt) encodes a protein of 579 aa with a predicted molecular mass of 65 kDa; this protein sequence shares similarities with the conserved domains of RNA-dependent RNA polymerases of other mycoviruses. Phylogenetic analysis indicated that AlRV1-HN28 was closely related to four other unclassified viruses, which suggests that the AlRV1-HN28 isolated from Alternaria longipes may belong to a new family of dsRNA mycoviruses. This is the first report of the full-length nucleotide sequence of a mycovirus that infects Alternaria longipes.