MR microscopy of cobalt-labeled nerve cells and pathways in an invertebrate brain (Sepia officinalis, Cephalopoda).
MR microscopy of cobalt-labeled nerve cells and pathways in an invertebrate brain (Sepia officinalis, Cephalopoda).
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无脊椎动物大脑（乌贼、头足类）中钴标记神经细胞和通路的 MR 显微镜检查。
DOI：
10.1002/mrm.1078
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发表时间：
2001
影响因子：
3.3
通讯作者：
Budelmann,BU
中科院分区：
文献类型：
--
作者：
Quast,MJ;Neumeister,H;Ezell,EL;Budelmann,BU
本文描述了对比增强MR显微镜的一种新应用，以追踪神经细胞和通过小型无脊椎动物大脑的通路。以乌贼Sepia officinalis（Cephalopoda）为模型，采用常规的向心钴离子导入法，用顺磁性钴离子标记乌贼脑神经的细胞和通路。在MR显微镜下，钴标记的细胞体和通路在9.4 T自旋回波图像中变为低信号。它们的病程和分布与硫化钴沉淀后常规组织学技术观察到的相同（有或无随后的银强化）。Magn Reson Med 45：575-579，2001。© 2001 Wiley利斯公司
This article describes a novel application of contrast‐enhanced MR microscopy to trace nerve cells and pathways through small invertebrate brains. Using the cuttlefishSepia officinalis(Cephalopoda) as a model, the cells and pathways of one of the brain nerves were labeled with paramagnetic cobalt(II) ions by conventional centripetal cobalt iontophoresis. In MR microscopy, the cobalt‐labeled cell bodies and pathways became hypointense in 9.4 T spin echo images. Their course and distribution were identical with those seen with conventional histological techniques after cobalt sulphide precipitation (with or without subsequent silver intensification). Magn Reson Med 45:575–579, 2001. © 2001 Wiley‐Liss, Inc.