THE FUNCTIONS OF TRYPTOPHAN RESIDUES IN MEMBRANE-PROTEINS
THE FUNCTIONS OF TRYPTOPHAN RESIDUES IN MEMBRANE-PROTEINS
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DOI：
10.1093/protein/5.3.213
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发表时间：
1992-04-01
期刊：
影响因子：
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通讯作者：
STEVENS, FJ
中科院分区：
文献类型：
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作者：
SCHIFFER, M;CHANG, CH;STEVENS, FJ
膜蛋白的色氨酸含量明显高于可溶性蛋白。紫色细菌光合反应中心的M和L亚基尤其如此。Trp残基不均匀地分布在膜上，而是集中在复合物的周质侧。此外，Trp残基不是随机排列的。在蛋白质亚基中，许多亚基与主链的羰基氧形成氢键，从而稳定蛋白质。在分子的表面上，它们被正确地定位以与脂质头部基团形成氢键，而它们的疏水环浸入双层的脂质部分中。这些观察结果表明，色氨酸残基参与蛋白质通过膜的易位，易位后，色氨酸残基作为锚定在膜的周质侧。
Membrane proteins have a significantly higher Trp content than do soluble proteins. This is especially true for the M and L subunits of the photosynthetic reaction center from purple bacteria. The Trp residues are not uniformly distributed through the membrane but are concentrated at the periplasmic side of the complex. In addition, Trp residues are not randomly aligned. Within the protein subunits, many form hydrogen bonds with carbonyl oxygens of the main chain, thereby stabilizing the protein. On the surface of the molecule, they are correctly positioned to form hydrogen bonds with the lipid head groups while their hydrophobic rings are immersed in the lipid part of the bilayer. These observations suggest that Trp residues are involved in the translocation of protein through the membrane and that following translocation, Trp residues serve as anchors on the periplasmic side of the membrane.