Deformability of breast cancer cells in correlation with surface markers and cell rolling
Deformability of breast cancer cells in correlation with surface markers and cell rolling
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DOI：
10.1096/fj.201700762r
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发表时间：
2017-11
期刊：
影响因子：
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通讯作者：
Amina Mohammadalipour;M. Burdick;D. Tees
中科院分区：
文献类型：
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作者：
Amina Mohammadalipour;M. Burdick;D. Tees
虽然癌症干细胞（CSC）假说已经存在多年，但细胞表面标记物对CSC分类的可靠性仍然存在争议。癌细胞比健康细胞更易变形的发现，为考虑将机械特性作为干性的可能生物标志物提供了动力。在这项研究中，使用微管抽吸技术，研究了多种乳腺癌细胞系的机械特性，并将其与乳腺癌干细胞（BCSC）标志物CD 44 +/CD 24 −/ALDH 1+相关联。结果表明，具有CD 44 +/CD 24 −/ALDH 1+表型的Hs 578 T和MDA-MB-231细胞系比不表达BCSC标志物的MDA-MB-468细胞系显著更易变形。具有中等变形能力的BT-20细胞系不表达任何CD 44 +/CD 24 −表型，但表达醛脱氢酶-1活性。此外，在平行板流动室中，发现更多可变形的细胞系在E选择素包被的基底上以剪切无关的速度滚动，这可能是转移期间CSC与内皮牢固粘附的介导因素。我们的研究结果表明，流变学特性可以被认为是除了表面标志物之外的生物力学标志物，或作为表面标志物的补充，以找到肿瘤内CSC特征的更明确证据。Mohammadyour，A.，Burdick，M. M.，Tees，D. F. J.乳腺癌细胞的变形性与表面标志物和细胞滚动相关。FASEB J. 32，1806-1817（2018）。www.fasebj.org
Although the cancer stem cell (CSC) hypothesis has been around for many years, the reliability of cellsurface markers to classify CSCs has remained debatable. The finding that cancerous cells are significantly more deformable than healthy ones has provided motivation to consider mechanical properties as a possible biomarker for stemness. In this study, using the micropipette aspiration technique, mechanical properties of multiple breast cancer cell lines were investigated and correlated with breast cancer stem cell (BCSC) marker, CD44+/CD24−/ALDH1+. The results indicated that Hs578T and MDA‐MB‐231 cell lines with CD44+/CD24−/ALDH1+ phenotype were significantly more deformable than the MDA‐MB‐468 cell line, which did not express the BCSC marker. The BT‐20 cell line with intermediate deformability did not express any CD44+/CD24− phenotype, but it expressed aldehyde dehydrogenase‐1 activity. In addition, more‐deformable cell lines were found to roll with shear‐independent velocities on E‐selectin‐coated substrates in a parallel‐plate flow chamber, which might be a mediating factor for firm adhesion of CSCs to endothelium during metastasis. Our results indicate that rheological properties can be considered as a biomechanical marker in addition to, or as a complement of, surface markers to find more‐definitive evidence of CSC characteristics within tumors.— Mohammadalipour, A., Burdick, M. M., Tees, D.F.J. Deformability of breast cancer cells in correlation with surface markers and cell rolling. FASEB J. 32, 1806–1817 (2018). www.fasebj.org