Characterization of p24 Gene of Spodoptera litura Multicapsid Nucleopolyhedrovirus
Characterization of p24 Gene of Spodoptera litura Multicapsid Nucleopolyhedrovirus
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DOI：
10.1007/s11262-004-6778-8
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发表时间：
2004-04
期刊：
影响因子：
1.6
通讯作者：
Zhaofei Li;Cui Li;Lijing Pan;Mei Yu;Kai Yang;Y. Pang
中科院分区：
文献类型：
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作者：
Zhaofei Li;Cui Li;Lijing Pan;Mei Yu;Kai Yang;Y. Pang
斜纹夜蛾核型多角体病毒(SpltMNPV)p24基因全长753bp，编码244个氨基酸，预测分子量为27.3 kDa。同源性分析表明，SpltMNPV P24与其他已知杆状病毒的氨基酸同源性为20-36%。RT-PCR结果表明，p24基因在感染后期转录活跃，其mRNA起始点位于杆状病毒晚期启动子一致序列(ATAAG)内。SpltMNPV感染SpltMNPV提取物的蛋白质印迹分析斜纹夜蛾细胞检测到一种特殊的28 kDa蛋白，该蛋白没有N-糖基化。结构定位表明，SpltMNPV P24与ODV的核衣壳以83 kDa的复合体形式结合。
Spodoptera lituramulticapsid nucleopolyhedrovirus (SpltMNPV)p24 gene is 753 bp long, potentially encoding 244 amino acids with a predicted molecular weight of 27.3 kDa. Homology analysis indicated that SpltMNPV P24 has 20–36% amino acid identity with that of other known baculoviruses. RT-PCR results showed that thep24 gene is transcribed actively at the late stage of infection and the mRNA start site was mapped within a consensus baculovirus late promoter sequence (ATAAG). Western blot analysis of extracts from SpltMNPV-infectedS. lituracells detected a specific 28 kDa protein, and this protein was notN-glycosylated. Structural localization revealed that SpltMNPV P24 was associated with the nucleocapsid of occlusion-derived virus (ODV) as a complex form of 83 kDa.