Complete mitochondrial DNA sequences of six snakes: Phylogenetic relationships and molecular evolution of genomic features
Complete mitochondrial DNA sequences of six snakes: Phylogenetic relationships and molecular evolution of genomic features
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DOI：
10.1007/s00239-004-0190-9
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发表时间：
2005-07-01
影响因子：
3.9
通讯作者：
Kumazawa, Y
中科院分区：
文献类型：
--
作者：
Dong, S;Kumazawa, Y
完整的线粒体DNA（mtDNA）序列测定的代表性物种从六个蛇类：acrochordid小文件蛇，大胆的蟒蛇，cylindrophiid红管蛇，蝰蛇himehabu，蟒蛇球蟒，和xenopeltid日光蛇。在这些mtDNA中鉴定出13个蛋白质编码基因、22个tRNA基因、2个rRNA基因和2个控制区。控制区的重复和tRNA（Leu）基因的易位是蛇mtDNA的两个显著特征。重复的控制区有几乎相同的核苷酸序列的物种内，但他们是不同的物种，这表明协调序列的两个控制区的进化。此外，重复的控制区似乎促进了蝰蛇科谱系中一些侧翼tRNA基因的交换。系统发育分析使用了大量的位点（9570个位点）来自完整的mtDNA序列。我们的数据有力地表明了一个新的系统发育关系的主要家庭的蛇：（蝰蛇科，游蛇科），Acrochordidae），（蟒蛇科，Xenopeltidae），蛇科），Boidae）），Leptotyphlopidae）。这一结论不同于一个广泛接受的观点的基础上的形态特征，否认姐妹集团的关系，boids和pythonids，以及基础分歧nonmacrostomatan cylindrophiids。这些结果为进一步利用mtDNA全序列等分子生物学数据重建蛇类系统发育提供了重要的理论依据。
Complete mitochondrial DNA (mtDNA) sequences were determined for representative species from six snake families: the acrochordid little file snake, the bold boa constrictor, the cylindrophiid red pipe snake, the viperid himehabu, the pythonid ball python, and the xenopeltid sunbeam snake. Thirteen protein-coding genes, 22 tRNA genes, 2 rRNA genes, and 2 control regions were identified in these mtDNAs. Duplication of the control region and translocation of the tRNA(Leu) gene were two notable features of the snake mtDNAs. The duplicate control regions had nearly identical nucleotide sequences within species but they were divergent among species, suggesting concerted sequence evolution of the two control regions. In addition, the duplicate control regions appear to have facilitated an interchange of some flanking tRNA genes in the viperid lineage. Phylogenetic analyses were conducted using a large number of sites (9570 sites in total) derived from the complete mtDNA sequences. Our data strongly suggested a new phylogenetic relationship among the major families of snakes: ((((Viperidae, Colubridae), Acrochordidae), (((Pythonidae, Xenopeltidae), Cylindrophiidae), Boidae)), Leptotyphlopidae). This conclusion was distinct from a widely accepted view based on morphological characters in denying the sister-group relationship of boids and pythonids, as well as the basal divergence of nonmacrostomatan cylindrophiids. These results imply the significance to reconstruct the snake phylogeny with ample molecular data, such as those from complete mtDNA sequences.