GRAIN-SORGHUM GLUTELIN - ISOLATION AND CHARACTERIZATION
GRAIN-SORGHUM GLUTELIN - ISOLATION AND CHARACTERIZATION
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DOI：
10.1021/jf60182a020
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发表时间：
1972-01-01
影响因子：
6.1
通讯作者：
BECKWITH, AC
中科院分区：
文献类型：
--
作者：
BECKWITH, AC
材料和方法盐酸胍（GHC 1，Eastman Organic Chemicals白色标签级）通过Kolthoff等人的方法重结晶。（一九五七年）。当在反应溶剂中使用时，通过Wu和Dimler（1964）的方法进一步纯化GHCl。3-巯基乙醇也购自Eastman Organic Chemicals，在氮气下再蒸馏。收集在153-155 ℃沸腾的馏分并用作二硫键还原剂.使用丙烯腈（Eastman黄色标记）作为巯基封闭剂，无需进一步纯化。Sephadex G-150交联葡聚糖购自Pharmacia Fine Chemical Inc.。二次重结晶的猪胰腺α-淀粉酶来自Mann研究实验室。酶的活性
MATERIALS AND METHODSGuanidine hydrochloride (GHC1, Eastman Organic Chemicals white label grade) was recrystallized by the method of Kolthoff et al.(1957). When used in reaction solvents, GHC1 was further purified by the procedure of Wu and Dimler (1964)./3-Mercaptoethanol, also purchased from Eastman Organic Chemicals, was redistilled under nitrogen. The fraction boiling at 153-155 C was collected and used as a disulfide bond reducing agent. Acrylonitrile (Eastman’s yellow label) was used as a sulfhydryl blocking agent without further purification. Sephadex G-150 crosslinked dextran was purchased from Pharmacia Fine Chemical Inc. Twice-recrystallized hog pancreas a-amylase came from the Mann Research Laboratories. The stated activity of theenzyme