Self-reproduction of supramolecular giant vesicles combined with the amplification of encapsulated DNA
Self-reproduction of supramolecular giant vesicles combined with the amplification of encapsulated DNA
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DOI：
10.1038/nchem.1127
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发表时间：
2011-10-01
期刊：
影响因子：
21.8
通讯作者：
Sugawara, Tadashi
中科院分区：
文献类型：
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作者：
Kurihara, Kensuke;Tamura, Mieko;Sugawara, Tadashi
从材料的角度构建原始细胞对于理解生命的起源非常重要。隔室的自我复制和信息物质的自我复制都已被广泛研究，但这些过程通常是独立进行的，而不是相互联系的。在这里，我们证明了DNA（封装的客人）内的自我再现的阳离子巨囊泡（主机）的扩增。随着囊泡膜前体的加入，我们观察到巨囊泡的生长和自发分裂，伴随着DNA向子巨囊泡的分布。特别是，DNA的扩增加速了巨大囊泡的分裂。这意味着信息物质的自我复制通过聚阴离子DNA和阳离子囊泡膜之间的相互作用与隔室的自我复制相关联。因此，我们的自我复制巨囊泡系统代表了构建先进模型原始细胞的一个进步。
The construction of a protocell from a materials point of view is important in understanding the origin of life. Both self-reproduction of a compartment and self-replication of an informational substance have been studied extensively, but these processes have typically been carried out independently, rather than linked to one another. Here, we demonstrate the amplification of DNA (encapsulated guest) within a self-reproducible cationic giant vesicle (host). With the addition of a vesicular membrane precursor, we observe the growth and spontaneous division of the giant vesicles, accompanied by distribution of the DNA to the daughter giant vesicles. In particular, amplification of the DNA accelerated the division of the giant vesicles. This means that self-replication of an informational substance has been linked to self-reproduction of a compartment through the interplay between polyanionic DNA and the cationic vesicular membrane. Our self-reproducing giant vesicle system therefore represents a step forward in the construction of an advanced model protocell.