Association of Superoxide‐Induced Oxidative Stress With Rotator Cuff Tears in Human Patients
Association of Superoxide‐Induced Oxidative Stress With Rotator Cuff Tears in Human Patients
复制标题
DOI：
10.1002/jor.24472
复制
发表时间：
2020-01
期刊：
影响因子：
--
通讯作者：
Keiichi Yoshida;Y. Itoigawa;Tomoki Wada;Y. Maruyama;H. Nojiri;T. Kawasaki;K. Kaneko
中科院分区：
文献类型：
--
作者：
Keiichi Yoshida;Y. Itoigawa;Tomoki Wada;Y. Maruyama;H. Nojiri;T. Kawasaki;K. Kaneko
肩袖退变是导致肩袖撕裂的因素之一。氧化应激参与肌腱退变，超氧化物诱导的氧化应激在调节氧化和还原之间的平衡中起病理作用。然而，氧化应激在肩袖撕裂中的作用尚不清楚。因此，本研究旨在研究超氧化物诱导的氧化应激对肩袖撕裂的影响。招募了70名患者，并将其分为两组：肩袖撕裂患者（破裂组）和未撕裂患者（未破裂组）。在手术过程中收集两组的标本。根据变性评分对变性形态进行分类。根据二氢乙锭（DHE）的相对荧光强度、根据超氧化物歧化酶（SOD）活性的抗氧化能力以及基于氧化还原比的氧化和还原之间的平衡来评估超氧化物诱导的氧化应激。比较两组数据。计算变性评分和氧化应激因子之间的相关性。破裂组的变性评分和DHE相对荧光强度显著高于未破裂组。各组间SOD活性无显著差异。变性评分与DHE相对荧光强度和SOD活性均呈正相关。因此，肩袖撕裂组织中的超氧化物诱导的氧化应激和肌腱变性大于无撕裂组织，表明氧化失衡可能与退行性肩袖撕裂有关。临床相关性：了解超氧化物诱导的氧化应激机制可能会导致退行性肩袖撕裂的靶向组织治疗。© 2019骨科研究学会。出版社：Wiley Periodicals，Inc. J Orthop Res 38：212-218，2020
Rotator cuff degeneration is one of the factors contributing to rotator cuff tears. Oxidative stress is involved in tendon degeneration, and superoxide‐induced oxidative stress plays a pathological role in regulating the balance between oxidation and reduction. The role of oxidative stress in rotator cuff tears, however, is unclear. This study, therefore, aimed to investigate the contribution of superoxide‐induced oxidative stress to rotator cuff tears. Seventy patients were recruited and divided into two groups: patients with (Ruptured group) and those without (Unruptured group) a rotator cuff tear. Specimens from both groups were collected during surgery. Degeneration morphology was classified according to the degeneration score. Superoxide‐induced oxidative stress was assessed according to dihydroethidium (DHE) relative fluorescence intensity, capacity for antioxidation according to superoxide dismutase (SOD) activity, and the balance between oxidation and reduction based on the redox ratio. Data were compared between groups. Correlations between the degeneration score and the oxidative stress factors were calculated. Degeneration score and DHE relative fluorescence intensity were significantly higher in the Ruptured than the Unruptured group. The SOD activity was not significantly different between groups. Degeneration score was positively correlated with both DHE relative fluorescence intensity and SOD activity. Thus, superoxide‐induced oxidative stress and tendon degeneration were greater in rotator cuff tear tissues than in those with no tear, suggesting that oxidative imbalance may be involved in degenerative rotator cuff tears. Clinical Relevance: Understanding the mechanisms of superoxide‐induced oxidative stress may lead to targeted tissue therapy for degenerative rotator cuff tears. © 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 38:212–218, 2020