Loss of IrpT Function in Lactococcus lactis subsp. lactis N8 Results in Increased Nisin Resistance
Loss of IrpT Function in Lactococcus lactis subsp. lactis N8 Results in Increased Nisin Resistance
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DOI：
10.1007/s00284-010-9615-4
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发表时间：
2010-03
影响因子：
2.6
通讯作者：
Z. Xuanyuan;Zhenzhou Wu;Ruiqing Li;D. Jiang;J. Su;Haijin Xu;Yanling Bai;Xiuming Zhang;P. Saris;M. Qiao
中科院分区：
文献类型：
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作者：
Z. Xuanyuan;Zhenzhou Wu;Ruiqing Li;D. Jiang;J. Su;Haijin Xu;Yanling Bai;Xiuming Zhang;P. Saris;M. Qiao
The antibiotic nisin, produced byLactococcus lactissubsp.lactisN8, offers an extensive commercial prospect as natural food preservatives. The nisin immunity of theL. lactisstrains is regulated by a variety of mechanisms. In this study, we isolated aL. lactisL31 strain with increased nisin resistance from a mini-Mu transposon mutant pool of strain N8. The single Mu insertion in strain L31 was in theirpTgene with unknown function. By comparing the proteomic profiles ofL. lactisL31 and its parental strain, we found that changes occurred in the synthesis of a protein involved in cell wall biosynthesis (RmlD). Strain L31 had 13.7% higher content of rhamnose in the cell wall than the N8 strain. Overexpression of RmlD involved in the synthesis of dTDP-l-rhamnose in the nisin-sensitive MG1363 strain increased nisin resistance of the strain. The results indicate that these cellular proteins effected nisin resistance inL.lactisN8.
