Antibiotic Resistance Genes and Taxa Analysis from Mat and Planktonic Microbiomes of Antarctic Perennial Ice-covered Lake Fryxell and Lake Bonney
Antibiotic Resistance Genes and Taxa Analysis from Mat and Planktonic Microbiomes of Antarctic Perennial Ice-covered Lake Fryxell and Lake Bonney
复制标题
DOI：
10.1017/s0954102022000360
复制
发表时间：
2022-06
期刊：
影响因子：
--
通讯作者：
Sheetal Tallada;Grant Hall;Daniel Barich;R. Morgan-Kiss;J. Slonczewski
中科院分区：
文献类型：
--
作者：
Sheetal Tallada;Grant Hall;Daniel Barich;R. Morgan-Kiss;J. Slonczewski
南极洲麦克默多干谷常年冰封的湖泊中存在着与其他水生系统地理上分离的贫营养微生物群落。它们的微生物组包括浮游微生物以及从冰中出现的升空垫群落。我们使用ShortBred来量化来自冰中出现的升空垫的宏基因组以及来自Fryxell湖和Bonney湖的过滤水样的抗生素抗性基因（ARG）。ARG命中的总体比例类似于在温带农村水体中发现的适度人类投入。具体的ARGs表现出不同的分布为两个湖泊，垫与反渗透来源。宏基因组分类群的分布表明，垫光养生物主要由蓝藻或betaproteobacteria，而水柱光养生物主要是原生生物。从弗里克塞尔湖垫样品的betaproteobacterium Rhodoferax anastriticus的富集培养物显示出一种不寻常的垫形成表型，以前没有报道过这种物种。它的基因组没有显示出与β变形菌相关的ARG，但具有与少量假单胞菌成分一致的ARG。南极湖垫和水表现出特定的ARG独特的垫和水源，但总体ARG水平与温和的人类输入的温带水体相似。
The perennial ice-covered lakes of the Antarctic McMurdo Dry Valleys harbor oligotrophic microbial communities that are separated geographically from other aquatic systems. Their microbiomes include planktonic microbes as well as lift-off mat communities that emerge from the ice. We used ShortBRED to quantify the antibiotic resistance genes (ARGs) from metagenomes of lift-off mats emerging from ice, and from filtered water samples of Lake Fryxell and Lake Bonney. The overall proportion of ARG hits was similar to that found in temperate-zone rural water bodies with moderate human inputs. Specific ARGs showed distinct distributions for the two lakes, and for mat versus planktonic sources. Metagenomic taxa distributions showed that mat phototrophs consisted mainly of cyanobacteria or betaproteobacteria, whereas the water column phototrophs were mainly protists. An enrichment culture of the betaproteobacterium Rhodoferax antarcticus from a Lake Fryxell mat sample showed an unusual mat-forming phenotype not previously reported for this species. Its genome showed no ARGs associated with betaproteobacteria, but had ARGs consistent with a minor Pseudomonas component. The Antarctic lake mats and water showed specific ARGs distinctive to the mat and water sources, but overall ARG levels were similar to those of temperate water bodies with moderate human inputs.