A candidate reference method for determination of total protein in serum. I. Development and validation.
A candidate reference method for determination of total protein in serum. I. Development and validation.
复制标题
测定血清中总蛋白的候选参考方法。
DOI：
10.1093/clinchem/27.10.1642
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发表时间：
1981
影响因子：
9.3
通讯作者：
R. Schaffer
中科院分区：
文献类型：
--
作者：
B. Doumas;D. Bayse;R. Carter;T. Peters;R. Schaffer
We developed a candidate Reference Method for measuring total serum protein by use of the biuret reaction. The method involves a previously described biuret reagent (Clin. Chem. 21: 1159, 1975) and Standard Reference Material (SRM) 927 bovine albumin (National Bureau of Standards) as the standard. At 25 degrees C, color development for 30 or 60 min provides identical serum protein values. Glucose (up to 10 g/L) and bilirubin (up to 300 mg/L) do not interfere. Hemoglobin, at 3 g/L, increases apparent serum protein by 0.4 g/L. The presence of dextran in serum causes easily detected turbidity, but this interference can be eliminated by centrifuging the reaction mixture. Therapeutic concentrations of ampicillin, carbenicillin, penicillin, oxacillin, nafcillin, chloramphenicol, cephalothin, and methicillin in blood do not interfere, nor do triglycerides up to 10 g/L. Within-run and day-to-day standard deviations of the method are 0.1 and 0.4 g/L, respectively.
