Microsatellite markers for Vaccinium from EST and genomic libraries

Microsatellite markers for Vaccinium from EST and genomic libraries
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DOI：
10.1111/j.1471-8286.2005.01025.x
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发表时间：
2005-09-01
期刊：
MOLECULAR ECOLOGY NOTES
影响因子：
--
通讯作者：
Rowland, LJ
Rowland, LJ
中科院分区：
其他
文献类型：
--
作者：
Boches, PS;Bassil, NV;Rowland, LJ

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本研究从表达序列标签（EST）和越橘基因组文库中分离出30个微卫星位点。11份四倍体和1份二倍体伞房花参试材料在24个单位点SSR中每个位点的等位基因数为2 ~ 15个（平均值为8.16）。在一组12个种属（代表9个切片）中的跨种属扩增范围为30 - 100%（平均值= 83%）。
We present 30 microsatellite loci isolated from expressed sequence tag (EST) and genomic libraries in Vaccinium corymbosum L. Allele number per locus in 11 tetraploid and one diploid V. corymbosum accessions ranged from two to 15 (mean = 8.16) in 24 single-locus simple sequence repeats (SSRs). Cross-species amplification in a panel of 12 species representing nine sections ranged from 30 to 100% (mean = 83%).