A role for B cells in the development of T cell helper function in a malaria infection in mice
A role for B cells in the development of T cell helper function in a malaria infection in mice
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DOI：
10.1073/pnas.95.4.1730
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发表时间：
1998-02-17
影响因子：
11.1
通讯作者：
von der Weid, T
中科院分区：
文献类型：
--
作者：
Langhorne, J;Cross, C;von der Weid, T
B细胞基因敲除小鼠无法清除查鲍迪疟原虫的原发红细胞感染。然而，早期的急性感染在一定程度上得到了控制，引起了慢性复发性寄生虫血症，可以通过药物治疗或过继转移B细胞来减少，类似于终生使用抗MU抗体治疗而导致B细胞缺陷的小鼠，B细胞敲除小鼠(Mu MT)在整个初次感染过程中对疟疾抗原保持主要的CD4(+)Th1样反应，这与对照C57BL/6小鼠的反应形成对比，在感染的后期，CD4(+)T细胞反应切换到Th2细胞的特征，显示出对体外特异性抗体和白细胞介素4产生的有效帮助。氯喹和过继转移免疫B细胞均可降低寄生虫载量，但过继转移B细胞可引起受体小鼠Th2应答，表现为产生抗体的辅助性细胞前体频率相对增加。这些数据支持B细胞在调节CD4(+)T细胞亚群反应中发挥作用的观点。
B cell knockout mice are unable to clear a primary erythrocytic infection of Plasmodium chabaudi chabaudi. However, the early acute infection is controlled to some extent, giving rise to a chronic relapsing parasitemia that can be reduced either by drug treatment or by adoptive transfer of B cells, Similar to mice rendered B-cell deficient by lifelong treatment with anti-mu antibodies, B cell knockout mice (mu MT) retain a predominant CD4(+) Th1-like response to malarial antigens throughout a primary infection, This contrasts with the response seen in control C57BL/6 mice in which the CD4(+) T-cell response has switched to that characteristic of Th2 cells at the later stages of infection, manifesting efficient help for specific antibodies in vitro and interleukin 4 production, Both chloroquine and adoptive transfer of immune B cells reduced parasite load, However, the adoptive transfer of B cells resulted in a Th2 response in recipient mu MT mice, as indicated by a relative increase in the precursor frequency of helper cells for antibody production. These data support the idea that B cells play a role in the regulation of CD4(+) T subset responses.