BACTERIAL INVASION IN ROOT CEMENTUM AND RADICULAR DENTIN OF PERIODONTALLY DISEASED TEETH IN HUMANS - A RESERVOIR OF PERIODONTOPATHIC BACTERIA
BACTERIAL INVASION IN ROOT CEMENTUM AND RADICULAR DENTIN OF PERIODONTALLY DISEASED TEETH IN HUMANS - A RESERVOIR OF PERIODONTOPATHIC BACTERIA
复制标题
DOI：
10.1902/jop.1988.59.4.222
复制
发表时间：
1988-04-01
影响因子：
4.3
通讯作者：
LOESCHE, WJ
中科院分区：
文献类型：
--
作者：
ADRIAENS, PA;DEBOEVER, JA;LOESCHE, WJ
本研究对牙周病无龋牙齿根状牙本质和牙髓内细菌的生存和分布进行了研究。健康牙齿作为对照。样本取自牙髓组织和根状牙本质。牙本质样本取自龈下区域的牙间表面。从牙髓侧开始，连续取样三至五层厚度约为1毫米的牙本质层。采用厌氧技术对样品进行处理和培养。87%的牙周病牙检出细菌生长。在83%的牙齿中，细菌至少存在于一个牙本质层中。在培养牙髓组织的患病牙齿中，59%的牙髓样本中含有细菌。牙髓和牙本质层的平均细菌浓度为每毫克组织1,399至16,537个菌落形成单位（CFU）。这些浓度是健康牙齿中浓度的259到7190倍。这表明牙周病牙的根部可能是细菌的宿主，机械处理后的牙根表面可能发生重新定植，以及牙髓感染。这些发现可能会改变目前关于牙周治疗中牙根表面清创的观念。
In this study the viability and the distribution of bacteria within the radicular dentin and pulp of periodontally diseased caries-free teeth were studied. Healthy teeth served as controls. Samples were obtained from the pulp tissue and from the radicular dentin. Dentin samples were taken from the interdental surfaces in the subgingival area. Starting from the pulpal side, three to five successive dentin layers of approximately 1 mm thickness were sampled. The samples were processed and cultured using an anaerobic technique. Bacterial growth was detected in 87% ofhte periodontally diseased teeth. In 83% of the teeth, bacteria were present in at least on of the dentin layers. Fifty-nine percent of the diseased teeth, from which the pulp tissue was cultured, contained bacteria n the pulp samples. The mean bacterial concentrations in the pulp and dentin layers ranged from 1,399 to 16,537 colony-forming units (CFU) per mg of tissue. These concentrations were 259 to 7,190 times greater than concentrations found in healthy teeth. It is suggested that the roots of periodontally diseased teeth could act as bacterial reservoirs from which recolonization of mechanically treated root surfaces can occur, as well as infection of the dental pulp. These findings might change current concepts concerning root surface debridement in periodontal therapy.