Comparisons of nucleotide sequences in the genomes of the New Jersey and Indiana serotypes of vesicular stomatitis virus
Comparisons of nucleotide sequences in the genomes of the New Jersey and Indiana serotypes of vesicular stomatitis virus
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新泽西州和印第安纳州水泡性口炎病毒血清型基因组核苷酸序列的比较
DOI：
10.1128/jvi.33.1.69-77.1980
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发表时间：
1980
影响因子：
5.4
通讯作者：
C. Pringle
中科院分区：
文献类型：
--
作者：
D. McGeoch;A. Dolan;C. Pringle
确定了新泽西血清型水疱性口炎病毒基因组RNA 3'末端附近和同一病毒N蛋白mRNA 3'末端聚腺苷酸链附近的约200个残基的核苷酸序列。将这些序列与先前确定的水泡性口炎病毒印第安纳血清型的相应序列进行比较。两个菌株获得的序列很容易比对，总体显示出 70.8% 的同源性。通过检查序列可以鉴定每种血清型 N mRNA 的翻译起始密码子和终止密码子。推导的两条N多肽的N端和C端氨基酸序列均相似，它们之间的大部分差异在于被明显同源的氨基酸取代。有人提出，这些核苷酸序列在其功能所施加的限制内，包含两种血清型基因组之间序列同源性程度的合理代表性测量，并且这高于先前估计的，但在扩展区域上几乎没有精确的同源性。
Nucleotide sequences of around 200 residues were determined adjacent to the 3' terminus of the genome RNA of vesicular stomatitis virus, New Jersey serotype, and adjacent to the 3'-terminal polyadenylic acid tract of the N protein mRNA of the same virus. These sequences were compared with the corresponding sequences previously determined for the Indiana serotype of vesicular stomatitis virus. The sequences obtained for the two strains were readily aligned, showing 70.8% homology overall. Examination of the sequences allowed identification of the translation initiation and termination codons for the N mRNA of each serotype. The deduced N-terminal and C-terminal amino acid sequences of the two N polypeptides were each similar, and most of the differences between them consisted of substitution by a clearly homologous amino acid. It was proposed that these nucleotide sequences, within limits imposed by their functions, comprise reasonably representative measures of the extent of sequence homology between the genomes of the two serotypes, and that this is higher than previously estimated, but with little exact homology over extended regions.