Mastoparan-induced hormone release from rat pancreatic islets.
Mastoparan-induced hormone release from rat pancreatic islets.
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Mastoparan 诱导大鼠胰岛释放激素。
DOI：
10.1210/endo.130.1.1727698
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发表时间：
1992
期刊：
影响因子：
4.8
通讯作者：
Takashi Yamada
中科院分区：
文献类型：
--
作者：
Mitsuhisa Komatsu;Toru Aizawa;Nakako Yokokawa;Yoshihiko Sato;Naomi Okada;Nobuyuki Takasu;Takashi Yamada
Mastoparan是从黄蜂毒液中纯化的十四肽，以剂量相关的方式刺激大鼠胰岛释放胰岛素和胰高血糖素。在灌注实验中，mastoparan产生双相激素释放，其在去除肽后10分钟内停止。暴露于mastoparan后，葡萄糖诱导的胰岛素释放明显保留。在孵育实验中，通过用百日咳毒素或新霉素（磷酸肌醇转换的抑制剂）预处理胰岛或通过将环境温度降低至17 ℃，mastoparan诱导的胰岛素释放被极大地阻断。用硝苯地平（钙通道阻滞剂）、H-7（A-和C-激酶抑制剂）、生长抑素或二价无阳离子培养基预处理胰岛不影响对mastoparan的反应。用对溴苯酰溴（磷脂酶A2抑制剂）预处理不会阻断高浓度（58 μ M）mastoparan诱导的反应。肽在30分钟孵育期间不刺激胰岛素合成。在正常（1.9 mM）和非常低（6.5 μ M）的细胞外Ca 2+浓度下，Mastoparan可升高分离的胰岛细胞中通过Fura-2测定的胞质游离Ca 2+浓度。大鼠静脉注射mastoparan可导致胰岛素和胰高血糖素显著升高。结合以前的数据，我们得出结论，mastoparan通过百日咳毒素敏感性GTP结合蛋白介导的温度依赖性过程刺激胰岛激素释放。磷脂酶-C的激活和细胞内Ca 2+的释放可能与胞吐作用耦合。通过Ca ~（2+）通道和蛋白激酶-A和-C的Ca ~（2+）内流似乎不参与mastoparan的释放作用。磷脂酶A2可能参与低浓度而非高浓度肽诱导的激素释放。
Mastoparan, a tetradecapeptide purified from wasp venom, stimulates insulin and glucagon release by rat pancreatic islets in a dose-related manner. In perifusion experiments, mastoparan produces monophasic hormone release, which ceases within 10 min of removal of the peptide. After exposure of the isles to mastoparan, glucose-induced insulin release is clearly retained. In incubation experiments, mastoparan-induced insulin release is greatly blocked by pretreatment of the islets with pertussis toxin or neomycin (inhibitor of phosphoinositide turnover) or by lowering the ambient temperature to 17 C. Pretreatment of the islets with nifedipine (calcium channel blocker), H-7 (inhibitor of A- and C-kinase), somatostatin, or divalent cation-free medium does not affect the response to mastoparan. Pretreatment with parabromophenacylbromide (phospholipase-A2 inhibitor) does not block the response induced by a high concentration of (58 microM) mastoparan. The peptide does not stimulate insulin synthesis during 30 min of incubation. Mastoparan raises the cytosolic free Ca2+ concentration, measured by fura-2, in isolated islet cells at normal (1.9 mM) and very low (6.5 microM) extracellular Ca2+ concentrations. Intravenous administration of mastoparan in rats causes a significant elevation of both insulin and glucagon. Together with the previous data, we conclude that mastoparan stimulates islet hormone release through a temperature-dependent process mediated by pertussis toxin-sensitive GTP-binding protein(s). Activation of phospholipase-C and liberation of intracellular Ca2+ are likely to be coupled to exocytosis. Ca2+ influx through the Ca2+ channel and protein kinase-A and -C appear not to be involved in mastoparan's hormone-releasing action. Phospholipase-A2 may be involved in the hormone release induced by low, but not high, concentrations of the peptide.
Mastoparan 刺激分离的肺泡 2 型细胞分泌表面活性剂的机制。
DOI：
--
发表时间：
1991
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Joyce-Brady,M;Rubins,JB;Panchenko,MP;Bernardo,J;Steele,MP;Kolm,L;Simons,ER;Dickey,BF
通讯作者：
Dickey,BF
促甲状腺激素释放激素、血管活性肠肽、佛波酯和 GH4C1 大鼠垂体细胞去极化的不同作用。
影响因子：
4.8
作者：
Aizawa,T;Hinkle,PM
通讯作者：
Hinkle,PM
促甲状腺激素释放激素快速刺激 GH4C1 大鼠垂体肿瘤细胞催乳素和生长激素的双相分泌。
影响因子：
4.8
作者：
Aizawa,T;Hinkle,PM
通讯作者：
Hinkle,PM