Correlation between Skeletal Muscle Calpain Activity and Fractional Rate of Muscle Degradation of Japanese Quail Coturnix coturnix japonica
Correlation between Skeletal Muscle Calpain Activity and Fractional Rate of Muscle Degradation of Japanese Quail Coturnix coturnix japonica
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鹌鹑骨骼肌钙蛋白酶活性与肌肉退化率的相关性
DOI：
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
T. Hashiguchi
中科院分区：
文献类型：
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作者：
K. Kawabe;Y. Maeda;S. Okamoto;T. Hashiguchi
本研究旨在阐明钙蛋白酶（EC 3. 4.22. 17)和肌肉蛋白降解系统。用N-甲基组氨酸法测定肌肉蛋白降解速率（Kd），用DEAE-Sephacel和Phenyl-Sepharose柱层析分离钙蛋白酶和钙蛋白酶抑制素，并以碱变性酪蛋白为底物测定其活性。Kd值与calpain和calpastatin活性的相关系数分别为0.728和-0.453。肌肉蛋白降解率（Y）与钙蛋白酶活性（X1）和钙蛋白酶抑制素活性（X2）的多元回归方程为Y=5.892+14.033X1-88.128X2（R2=0.706）。Calpain和Calpastatin与Kd的标准化偏回归系数分别为0.605和-0.453。这些值表明：1）钙蛋白酶和钙蛋白酶抑制剂的活性影响肌肉蛋白质降解速率，2）肌肉中钙蛋白酶或钙蛋白酶抑制剂活性的个体信息可以作为蛋白质周转和生长的选择特征。（Jpn. Poult.科学，34：231-239，1997）
The present study was conducted to clarify the relationship between calpain (EC 3. 4.22. 17) and muscle protein degradation system using Japanese quail. The fractional rates of muscle protein degradation (Kd) were determined by the Nz-methylhistidine methods, and both of calpain and calpastatin were separated by DEAE-Sephacel and Phenyl-Sepharose column chromatography, and measured their activities with alkali denatured casein as a substrate. The correlation coefficients between the Kd and the calpain or calpastatin activities were 0.728 and -0.453, respectively. Multiple regression equation of muscle protein degradation rate (Y) to calpain activity (X1) and calpastatin activity (X2) was calculated as Y=5.892+14.033X1-88.128X2 (R2=0.706). The standardized partial regression coefficients of calpain and calpastatin to Kd were 0.605 and -0.453, respectively. These values suggest that 1) activities of calpain and calpastatin affect the muscle protein degradation rate, and 2) individual information on calpain or calpastatin activities in muscle may be available as a selection characteristic for the protein turnover and growth. (Jpn. Poult. Sci., 34: 231-239, 1997)
遗传性小鼠肌营养不良症中的骨骼肌蛋白质和氨基酸代谢。
DOI：
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发表时间：
1980
期刊：
The Journal of biological chemistry
影响因子：
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作者：
Garber,AJ;Schwartz,RJ;Seidel,CL;Silvers,A;Entman,ML
通讯作者：
Entman,ML