Deletion of cell division cycle 2-like 1 gene locus on 1p36 in non-Hodgkin lymphoma
Deletion of cell division cycle 2-like 1 gene locus on 1p36 in non-Hodgkin lymphoma
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DOI：
10.1016/s0165-4608(98)00138-1
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发表时间：
1999-01-15
影响因子：
--
通讯作者：
Sanger, WG
中科院分区：
文献类型：
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作者：
Dave, BJ;Pickering, DL;Sanger, WG
我们的实验室记录了非霍奇金淋巴瘤（NHL）中染色体1p36重排的显著高发生率。细胞分裂周期2-样1(CDC2L1)（也称为TP58或PITSLRE）基因是一种与凋亡信号有关的蛋白激酶，位于染色体1p36的非常远端区域，可能被涉及1p36的结构重排破坏。为了确定1p36区域复发累及的分子后果，我们使用TP58(clk-1) DNA探针，通过荧光原位杂交（FISH）检测了来自26例患者的31个标本中含有1p36异常的中期CDC2L1的可能缺失。23例出现异常1号染色体CDC2L1缺失。在26例病例中，2例基因位点易位到伴侣染色体上，4例标本（均来自同一病例）未缺失CDC2L1。这项初步研究表明，1p36重排以及由此导致的CDC2L1基因位点的缺失在NHL中很重要。这项工作也为进一步的分子研究和预后相关性开辟了道路。(C) Elsevier Science Inc., 1998。
Our laboratories have documented a significantly high occurrence of chromosome 1p36 rearrangements in non-Hodgkin lymphoma (NHL). The cell division cycle 2-like 1(CDC2L1) (also known as TP58 or PITSLRE) gene, a protein kinase implicated in apoptotic signaling, is located at the very distal region of chromosome 1p36 and is likely to be disrupted by structural rearrangements involving 1p36. To determine the molecular consequences of the recurrent involvement of the 1p36 region, we examined metaphases containing 1p36 abnormalities from 31 specimens derived from 26 patients for the possible deletion of CDC2L1 by fluorescence in situ hybridization (FISH) using the TP58(clk-1) DNA probe. Twenty-three cases exhibited the loss of CDC2L1 from the abnormal chromosome 1. In 2 of 26 cases, the gene locus was translocated to the partner chromosome, and in four specimens, all derived from one case, CDC2L1 was not deleted. This pilot investigation suggests that 1p36 rearrangements, and consequently the loss of the CDC2L1 gene locus, is important in NHL. This work also opens avenues for further molecular studies and prognostic correlations. (C) Elsevier Science Inc., 1998.