EXPRESSION OF ROD AND CONE VISUAL PIGMENTS IN GOLDFISH AND ZEBRAFISH - A RHODOPSIN-LIKE GENE IS EXPRESSED IN CONES
EXPRESSION OF ROD AND CONE VISUAL PIGMENTS IN GOLDFISH AND ZEBRAFISH - A RHODOPSIN-LIKE GENE IS EXPRESSED IN CONES
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DOI：
10.1016/0896-6273(93)90064-x
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发表时间：
1993-06-01
期刊：
影响因子：
16.2
通讯作者：
KNIGHT, JK
中科院分区：
文献类型：
--
作者：
RAYMOND, PA;BARTHEL, LK;KNIGHT, JK
本研究的主要目的是确定视紫红质样基因是否实际上在视锥感光细胞而不是视杆细胞中表达，该基因被认为在几个物种中代表绿色视锥色素。用比色原位杂交的方法研究了视杆蛋白和蓝、红视锥蛋白在金鱼和斑马鱼视网膜中的表达模式。结果表明，视紫红质样蛋白基因如预测的那样在绿色视锥细胞中表达。不与这些cRNA探针杂交的小锥体的子集被初步鉴定为紫外线受体。结果还表明，视蛋白信息在视锥细胞中仅限于核周区域，而在视杆细胞中，视蛋白信息既在核周又在椭球附近。
The primary purpose of the present study was to determine whether a rhodopsin-like gene, which has been postulated to represent the green cone pigment in several species, is in fact expressed in cone photoreceptors instead of rods. The expression patterns of rod opsin and blue and red cone opsins were also examined in both goldfish and zebrafish retinas using colorimetric in situ hybridization. The results demonstrate that the rhodopsin-like gene is expressed in green cones, as predicted. A subset of small cones that do not hybridize with these cRNA probes are tentatively identified as ultraviolet receptors. The results also demonstrate that opsin message in cones is restricted to the perinuclear region, whereas in rods, it is both perinuclear and adjacent to the ellipsoid.