SUPERFICIALLY POROUS SILICA MICROSPHERES FOR THE FAST HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF MACROMOLECULES
SUPERFICIALLY POROUS SILICA MICROSPHERES FOR THE FAST HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF MACROMOLECULES
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DOI：
10.1021/ac00035a009
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发表时间：
1992-06-01
影响因子：
7.4
通讯作者：
KIRKLAND, JJ
中科院分区：
文献类型：
--
作者：
KIRKLAND, JJ
合成了一种新型的表面多孔“孔壳”颗粒，并对其进行了高效液相色谱分离实验。喷雾干燥技术用于制备二氧化硅微球，通常具有5mu -m的固体核心和大约1mu -m厚的外壳，具有300埃的孔。与相同尺寸的常规全多孔颗粒相比，孔壳颗粒表现出更好的传质动力学特性，可以更快地分离大分子。新的Poroshell柱填料的实用性被证明与蛋白质和合成聚合物的分离。
New superficially porous "Poroshell" particles have been synthesized and tested for HPLC separations of macromolecules. Spray-drying techniques were used to prepare silica microspheres that typically have a 5-mu-m solid core and a approximately 1-mu-m-thick outer shell with 300-angstrom pores. Compared to conventional totally porous particles of the same size, Poroshell particles show improved mass-transfer kinetic properties that permit more rapid separations of macromolecules. The utility of the new Poroshell column packing is demonstrated with separations of proteins and synthetic polymers.