Survey of extrachromosomal DNA found in the filamentous cyanobacteria

Survey of extrachromosomal DNA found in the filamentous cyanobacteria
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丝状蓝藻染色体外 DNA 的调查

DOI:
10.1128/jb.136.1.414-418.1978
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发表时间:
1978
影响因子:
3.2
通讯作者:
R. Simon
R. Simon
中科院分区:
生物学3区
文献类型:
--
作者:
R. Simon

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用琼脂糖凝胶电泳和溴化乙锭染色法检测了13种丝状蓝藻的裂解液中染色体外DNA的存在。13个种中有7个含有染色体外共价闭合环状DNA,除1个种外均含有多个元件。extrachomosomal DNA的存在和在蓝藻中发现的代谢活动的范围或由形态复杂的丝状蓝藻阐述的分化细胞类型或结构之间没有相关性。
Cleared lysates of 13 species of filamentous cyanobacteria were examined for the presence of extrachromosomal DNA by using agarose gel electrophoresis and ethidium bromide staining. Seven of the 13 species contained extrachromosomal covalently closed circular DNA, and all but 1 species contained multiple elements. There was no correlation between the presence of extrachomosomal DNA and either the range of metabolic activities found in the cyanobacteria or the differentiated cell types or structures elaborated by the morphologically complex filamentous cyanobacteria.