Mesothelial cell apoptosis is confirmed in vivo by morphological change in cytokeratin distribution.
Mesothelial cell apoptosis is confirmed in vivo by morphological change in cytokeratin distribution.
复制标题
间皮细胞凋亡在体内通过细胞角蛋白分布的形态变化得到证实。
DOI：
10.1152/ajplung.2000.278.3.l528
复制
发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Broaddus,VC
中科院分区：
文献类型：
--
作者：
Marchi,E;Liu,W;Broaddus,VC
间皮细胞的凋亡已在体外得到证实，但在体内尚未得到证实。为了将凋亡的胸膜细胞鉴定为间皮细胞，我们使用细胞角蛋白作为标记物，并在明显凋亡的间皮细胞中发现了细胞角蛋白的惊人球状聚集外观。在体外研究中，我们发现聚集的细胞角蛋白模式与小鼠、兔子和人类的原代间皮细胞的凋亡相关，并且没有观察到坏死。在小鼠体内研究中，我们使用这种细胞角蛋白模式来识别和定量凋亡间皮细胞。凋亡间皮细胞最好通过胸膜灌洗来收获，表明它们是松散贴壁或不贴壁的。滴注 RPMI 1640 培养基或硅灰石 24 小时，分别诱导 0.1 ± 0.1 (SE) 和 1.0 ± 0.7% 恢复的所有间皮细胞凋亡，而滴注已知的凋亡刺激物、青石棉 (25 μg) 24 小时或放线菌素 D 加鼠肿瘤坏死因子-α 12 小时，分别诱导 5.1 ± 0.5 和 22.4 ± 4.5% 的细胞凋亡（显着高于对照实验，P < 0.05）。通过细胞角蛋白染色分析，体内间皮细胞凋亡已得到证实。
Apoptosis of mesothelial cells has been demonstrated in vitro but not in vivo. To identify apoptotic pleural cells as mesothelial, we used cytokeratin as a marker and found a striking spheroid, aggregated appearance of cytokeratin in apparently apoptotic mesothelial cells. In in vitro studies, we found that the aggregated cytokeratin pattern correlated with apoptosis in primary mesothelial cells from mice, rabbits, and humans and was not seen with necrosis. In in vivo studies in mice, we then used this cytokeratin pattern to identify and quantitate apoptotic mesothelial cells. Apoptotic mesothelial cells were best harvested by pleural lavage, indicating that they were loosely adherent or nonadherent. Instillation of RPMI 1640 medium or wollastonite for 24 h induced apoptosis in 0.1 ± 0.1 (SE) and 1.0 ± 0.7%, respectively, of all mesothelial cells recovered, whereas instillation of known apoptotic stimuli, crocidolite asbestos (25 μg) for 24 h or actinomycin D plus murine tumor necrosis factor-α for 12 h, induced apoptosis in 5.1 ± 0.5 and 22.4 ± 4.5%, respectively (significantly greater than in control experiments, P < 0.05). By analysis of cytokeratin staining, mesothelial cell apoptosis has been confirmed in vivo.