Distribution and activity of a Dippu DH31-like peptide in the large milkweed bug Oncopeltus fasciatus
Distribution and activity of a Dippu DH31-like peptide in the large milkweed bug Oncopeltus fasciatus
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DOI：
10.1016/j.peptides.2007.10.031
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发表时间：
2008-02-01
期刊：
影响因子：
3
通讯作者：
Orchard, I.
中科院分区：
文献类型：
--
作者：
Brugge, V. A. Te;Orchard, I.
马利筋虫（Oncopeltus fasciatus）是一种喂养半翅目昆虫的植物。虽然已经有很多关于神经激素控制吸血半翅目昆虫，Rhodnius prolixus的摄食后利尿的研究，但对O.带条肌可能在这种快速利尿中发挥作用的神经激素之一属于降钙素样利尿激素（DH31）昆虫肽家族。在这项研究中，我们证明了存在的DH31样免疫反应在中枢神经系统（CNS）和肠道的O。条带绦虫5龄。同样，从5龄O的CNS中定量并部分纯化了DH31样物质。使用反相液相色谱法（RPLC）检测并用酶联免疫吸附测定法（ELISA）监测。在O.与盐水对照相比，DH31样肽显著增加了分泌速率。结果提示，在O. Fasciatus的，并且该肽可能在控制马氏管分泌中起作用。(c)2007年爱思唯尔公司All rights reserved.
The milkweed bug, Oncopeltus fasciatus, is a plant feeding hemipteran. While there has been much research done on the neurohormonal control of the post-feeding diuresis in the blood-feeding hemipteran, Rhodnius prolixus, little is known about the control of the post-feeding diuresis in O. fasciatus. One of the neurohormones that may play a role in this rapid diuresis belongs to the calcitonin-like diuretic hormone (DH31) family of insect peptides. In this study we demonstrate the presence of DH31-like immunoreactivity in the central nervous system (CNS) and gut of O. fasciatus 5th instars. As well, DH31-like material was quantified and partially purified from the CNS of 5th instar O. fasciatus using reversed-phase liquid chromatography (RPLC) and monitored with an enzyme-linked immunosorbent assay (ELISA). When tested on O. fasciatus 5th instar Malpighian tubules, DH31-like peptides significantly increased the rate of secretion over saline controls. The results suggest that there is a DH31-like peptide(s) present in the CNS of O. fasciatus and that this peptide may play a role in the control of Malpighian tubule secretion. (c) 2007 Elsevier Inc. All rights reserved.