Disease Resistance of Tissue-cultured Seedlings of Rhododendron after Treatment with Streptomyces sp. R-5
Disease Resistance of Tissue-cultured Seedlings of Rhododendron after Treatment with Streptomyces sp. R-5
复制标题
链霉菌处理杜鹃组培苗的抗病性。
DOI：
10.1007/pl00013040
复制
发表时间：
2001
影响因子：
1.2
通讯作者：
H. Kunoh
中科院分区：
文献类型：
--
作者：
M. Shimizu;Norikazu Fujita;Y. Nakagawa;T. Nishimura;T. Furumai;Y. Igarashi;H. Onaka;R. Yoshida;H. Kunoh
内生放线菌，链霉菌。 R-5是从田间杜鹃花植物中分离得到的，用于保护组织培养的杜鹃幼苗免受由Pestalotiopsis sydowiana引起的多盘盘菌病的侵害。R-5对多盘盘菌具有强烈的拮抗活性。 sydowiana 不会对玻璃烧瓶中的幼苗产生不利影响。将R-5的悬浮液铺展在玻璃烧瓶中的增殖培养基的表面上，其中幼苗在该玻璃烧瓶中生长。 10天后，在幼苗的第4片上部叶上接种P。 sydowiana并孵育14天。在未经R-5处理的对照中，底物菌丝体为P。接种后 2-3 天内，sydowianag 在第四片叶子上方和下方的所有叶子和茎上生长。这样的生长导致 14 天时 54% 的幼苗枯萎死亡。相比之下，只有接种的叶子变成棕色印加。 90% 的幼苗生长在经过 R-5 处理的培养基上。这些幼苗没有一株死亡。因此，用 R-5 处理培养基表面可有效保护幼苗免受 P 感染。 sydowiana.扫描电子显微镜显示R-5的基质菌丝体在处理过的幼苗的叶子角质层之上和之下生长。荧光显微镜显示 R-5 也在叶子内部。
An endophytic actinomycete,Streptomycessp. R-5, which had been isolated from a field-grown rhododendron plant, was used to protect rhododendron seedlings in tissue culture from Pestalotia disease caused byPestalotiopsis sydowiana.R-5 had intense antagonistic activity againstP. sydowianawithout adversely affecting the seedlings in glass flasks. A suspension of R-5 was spread on the surface of the multiplication medium in glass flasks in which seedlings were growing. Ten days later, the 4th upper leaf of seedlings was inoculated withP. sydowianaand incubated for 14 days. In controls untreated with R-5, substrate mycelia ofP. sydowianagrew on all leaves and stems above and below the 4th leaf within 2–3 days of inoculation. Such growth resulted in the wilting death of 54% of seedlings by 14 days. In contrast, only the inoculated leaves turned brown inca. 90% of seedlings growing on medium treated with R-5. None of these seedlings died. Thus, treatment of the medium surface with R-5 efficiently protects the seedlings from infection byP. sydowiana.Scanning electron microscopy revealed that substrate mycelia of R-5 grew on and beneath the cuticle of leaves of the treated seedlings. Fluorescent microscopy showed that R-5 was also inside the leaves.