MINUS-STRAND COPIES OF REPLICATING CORONAVIRUS MESSENGER-RNAS CONTAIN ANTILEADERS
MINUS-STRAND COPIES OF REPLICATING CORONAVIRUS MESSENGER-RNAS CONTAIN ANTILEADERS
复制标题
DOI：
10.1128/jvi.65.1.320-325.1991
复制
发表时间：
1991-01-01
影响因子：
5.4
通讯作者：
BRIAN, DA
中科院分区：
文献类型：
--
作者：
SETHNA, PB;HOFMANN, MA;BRIAN, DA
测定了猪传染性胃肠炎冠状病毒mRNA的5'前导序列，发现其长度为90个核苷酸。在对来自感染细胞的RNA进行北方分析时，使用具有前导序列内序列的寡脱氧核苷酸作为探针，并且显示出抗前导序列（前导序列的负链拷贝）存在于所有mRNA负链种类上。RNA酶保护分析表明，反前导序列的长度与前导序列的长度大致相同。反前导序列出现的动力学与负链RNA种类的出现相同。沿着病毒mRNA被包装的证据，进一步支持了冠状病毒mRNA可以通过亚基因组mRNA长度的复制中间体进行复制的观点，以及来自感染病毒体的输入mRNA可以作为其自身复制的初始模板。从这个意义上说，冠状病毒的行为部分类似于具有分段基因组的RNA病毒。
The 5' leader sequence on mRNAs of the porcine transmissible gastroenteritis coronavirus was determined and found to be 90 nucleotides in length. An oligodeoxynucleotide with a sequence from within the leader was used as a probe in Northern analysis on RNA from infected cells, and an antileader (a minus-strand copy of the leader sequence) was shown to be present on all mRNA minus-strand species. RNase protection analysis showed the antileader to be approximately the same length as the leader. The kinetics of antileader appearance was the same as that for the appearance of minus-strand RNA species. This, along with a demonstration that viral mRNAs become packaged, gives further support to the idea that coronavirus mRNAs can undergo replication via subgenomic mRNA-length replicative intermediates, and that input mRNAs from infecting virions may serve as initial templates for their own replication. In this sense, then, coronaviruses behave in part like RNA viruses with segmented genomes.