Identification and expression profile analysis of NUCLEAR FACTOR-Y families in Physcomitrella patens.
Identification and expression profile analysis of NUCLEAR FACTOR-Y families in Physcomitrella patens.
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小立碗藓核因子-Y 家族的鉴定和表达谱分析。
DOI：
10.3389/fpls.2015.00642
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发表时间：
2015
影响因子：
5.6
通讯作者：
He Y
中科院分区：
文献类型：
--
作者：
Zhang F;Han M;Lv Q;Bao F;He Y
核因子Y转录因子属于多成员家族，由NF-YA/B/C亚基组成。已经报道NF-Y家族的成员调节植物中的生理过程。在这项研究中，我们确定并注释了小立碗藓基因组中的2个NF-YA，9个NF-B和12个NF-YC蛋白。保守结构域分析表明，PpNF-YA/B/C与拟南芥、O. sativa和mouse。PpNF-Ys基因在展叶侧耳原丝体和配子体的不同组织和发育阶段均有表达。大多数PpNF-Y基因通过ABA非依赖或依赖途径响应非生物胁迫。ABA调节的PpNF-Y表达的一部分由ABI 3介导。本研究首次对小立碗藓中的NF-Y家族进行了分析，为进一步研究PpNF-Ys的功能奠定了基础。
NUCLEAR FACTOR Y transcription factors belong to a multimember family and consist of NF-YA/B/C subunits. Members of the NF-Y family have been reported to regulate physiological processes in plant. In this study, we identified and annotated two NF-YA, nine NF-B, and twelve NF-YC proteins in the genome of Physcomitrella patens. Analyses of conserved domains demonstrated that PpNF-YA/B/C shared the same conserved domains with their orthologous proteins in Arabidopsis, O. sativa and mouse. Expression profiles indicated that PpNF-Ys were widely expressed in different tissues and developmental stages of P. patens throughout protonema and gametophores. The majority of PpNF-Y genes were responsive to abiotic stress via either ABA-independent or -dependent pathways. Some of ABA-regulated PpNF-Y expression were mediated by ABI3. To our knowledge, this study was the first to evaluate NF-Y families in Physcomitrella patens, and provides a foundation to dissect the function of PpNF-Ys.