Automated, PCR-RFLP genotyping of the urokinase gene.
Automated, PCR-RFLP genotyping of the urokinase gene.
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尿激酶基因的自动化 PCR-RFLP 基因分型。
DOI：
10.1089/10906570050501551
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Booyse,F
中科院分区：
文献类型：
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作者：
Sell,SM;Blasi,F;Booyse,F
尿激酶型纤溶酶原激活物(u-PA)被认为在动脉粥样硬化和冠状动脉疾病(CAD)的早期发生和发展中起作用(Griettet al.，1998)。最近，u-PA基因非翻译区的一种常见的遗传多态被证明与症状性CAD有关。为了研究这种常见的遗传多态在u-PA基因中的可能作用，我们开发了一种基于PCR的自动化检测方法。尿激酶基因BamHI多态的PCR-RFLP基因分型的自动化将支持对进行必要的人群研究以揭示疾病易感性关联所需的大样本量的筛选。
The urokinase-type plasminogen activator (u-PA) has been suggested to play a role in the early initiation and progression of atherosclerosis and coronary artery disease (CAD) (Grenettet al., 1998). Recently, a common genetic polymorphism in the untranslated region of theu-PAgene was shown to be associated with syptomatic CAD. To study the possible role of this common genetic polymorphism in theu-PAgene, we have developed an automated, PCR-based assay. Automation of the PCR-RFLP genotyping of theBamHI polymorphism of the urokinase gene will support the screening of the large sample sizes required to do the population-based studies necessary to uncover disease susceptibility associations.