Production of indigo by recombinant bacteria.
Production of indigo by recombinant bacteria.
复制标题
通过重组细菌生产靛蓝。
DOI：
10.1186/s40643-023-00626-7
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发表时间：
2023
影响因子：
4.6
通讯作者：
中科院分区：
文献类型：
--
作者：
靛蓝是一种经济上重要的染料，特别是用于纺织工业和牛仔裤和服装的牛仔布织物的染色。每年约有8万吨靛蓝是通过使用不可再生的石化产品以及使用和产生有毒化合物而化学生产的。由于许多微生物及其酶能够在表达特定的加氧酶和羟化酶后合成靛蓝，微生物发酵可以提供一个更可持续和环境友好的制造平台。虽然已经进行了多项小规模的研究，但现有的一些研究空白仍然阻碍了这些生物化学方法的有效转化。没有一篇文章评估了靛蓝生物催化在实际工业应用中的可行性和相关性。没有关于靛蓝生物合成的已建立或实际测试的大规模生物工艺的记录。为了解决这个问题，对靛蓝生物合成进行了上游和下游加工考虑。确定了5类潜在的生物催化剂，并设计了2种可能的生物工艺流程图，以便于生成预还原染料溶液或干粉产品。此外，考虑到相关技术和常见生物工艺设施开发的公开数据，估计了可能的平台和工艺价值，包括滴度、DSP产率、潜在工厂产能、发酵罐规模和批次计划。这使我们能够预测潜在靛蓝生物合成平台的实际年产量为540吨。这被解释为一个工业相关的数量，足以提供一个工业规模的牛仔布染色厂每年的染料供应。进行的灵敏度分析表明，该预期输出对反应滴度的变化最为敏感，可导致27.8%的增加或94.4%的下降。因此，尽管这种生物平台在实际实施之前需要仔细考虑、微调和优化，但发现重组靛蓝生物合成对于奢侈品客户和牛仔服装的大规模生产商的商业开发已经具有吸引力。在线版本包含补充材料，可通过10.1186/s40643-023-00626-7获得。
Indigo is an economically important dye, especially for the textile industry and the dyeing of denim fabrics for jeans and garments. Around 80,000 tonnes of indigo are chemically produced each year with the use of non-renewable petrochemicals and the use and generation of toxic compounds. As many microorganisms and their enzymes are able to synthesise indigo after the expression of specific oxygenases and hydroxylases, microbial fermentation could offer a more sustainable and environmentally friendly manufacturing platform. Although multiple small-scale studies have been performed, several existing research gaps still hinder the effective translation of these biochemical approaches. No article has evaluated the feasibility and relevance of the current understanding and development of indigo biocatalysis for real-life industrial applications. There is no record of either established or practically tested large-scale bioprocess for the biosynthesis of indigo. To address this, upstream and downstream processing considerations were carried out for indigo biosynthesis. 5 classes of potential biocatalysts were identified, and 2 possible bioprocess flowsheets were designed that facilitate generating either a pre-reduced dye solution or a dry powder product. Furthermore, considering the publicly available data on the development of relevant technology and common bioprocess facilities, possible platform and process values were estimated, including titre, DSP yield, potential plant capacities, fermenter size and batch schedule. This allowed us to project the realistic annual output of a potential indigo biosynthesis platform as 540 tonnes. This was interpreted as an industrially relevant quantity, sufficient to provide an annual dye supply to a single industrial-size denim dyeing plant. The conducted sensitivity analysis showed that this anticipated output is most sensitive to changes in the reaction titer, which can bring a 27.8% increase or a 94.4% drop. Thus, although such a biological platform would require careful consideration, fine-tuning and optimization before real-life implementation, the recombinant indigo biosynthesis was found as already attractive for business exploitation for both, luxury segment customers and mass-producers of denim garments. The online version contains supplementary material available at 10.1186/s40643-023-00626-7.
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影响因子：
15
作者：
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通讯作者：
Finogenova, Tatiana V