Modified pyrimidines specifically bind the purine riboswitch
Modified pyrimidines specifically bind the purine riboswitch
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DOI：
10.1021/ja063645t
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发表时间：
2006-11-08
影响因子：
15
通讯作者：
Batey, Robert T.
中科院分区：
文献类型：
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作者：
Gilbert, Sunny D.;Mediatore, Sarah J.;Batey, Robert T.
嘌呤核糖开关是一种在革兰氏阳性菌 5'-非翻译区中发现的遗传调控元件，可特异性响应鸟嘌呤或腺嘌呤调节 mRNA 的表达。我们报道，腺嘌呤响应RNA元件还能够以类似于嘌呤化合物的方式特异性识别在6-或5,6-位上带有修饰的嘧啶化合物。使用等温滴定量热法和 X 射线晶体学，对这些化合物的结合进行了表征。
The purine riboswitch is a genetic regulatory element found in the 5‘-untranslated regions of Gram-positive bacteria that regulates expression of the mRNA specifically in response to either guanine or adenine. We report that the adenine-responsive RNA element is also capable of specifically recognizing pyrimidine compounds bearing modifications at the 6- or 5,6-positions in a fashion similar to that of purine compounds. Using isothermal titration calorimetry and X-ray crystallography, the binding of these compounds is characterized.