TRK1 ENCODES A PLASMA-MEMBRANE PROTEIN REQUIRED FOR HIGH-AFFINITY POTASSIUM-TRANSPORT IN SACCHAROMYCES-CEREVISIAE
TRK1 ENCODES A PLASMA-MEMBRANE PROTEIN REQUIRED FOR HIGH-AFFINITY POTASSIUM-TRANSPORT IN SACCHAROMYCES-CEREVISIAE
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DOI：
10.1128/mcb.8.7.2848
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发表时间：
1988-07-01
影响因子：
5.3
通讯作者：
FINK, GR
中科院分区：
文献类型：
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作者：
GABER, RF;STYLES, CA;FINK, GR
我们在酿酒酵母中鉴定出一种 180 千道尔顿的质膜蛋白，它是高亲和力转运（摄取）钾所需的。编码这种假定的钾转运蛋白 (TRK1) 的基因因其能够缓解 trk1 细胞中钾转运缺陷的能力而被克隆。 TRK1 编码一种长 1,235 个氨基酸的蛋白质，包含 12 个潜在的跨膜结构域。我们的结果证明了酵母钾和质子运输系统的物理和功能独立性。 TRK1 在酿酒酵母中不是必需的，并且定位到与 PMA1（编码质膜 ATP 酶的基因）无关的基因座。含有 TRK1 无效等位基因 (trk1.DELTA.) 的单倍体细胞依赖于低亲和力转运蛋白来摄取钾，并且在某些条件下，表现出能量依赖性钾损失，直接暴露出负责该离子流出的转运蛋白的活性。
We identified a 180-kilodalton plasma membrane protein in Saccharomyces cerevisiae required for high-affinity transport (uptake) of potassium. The gene that encodes this putative potassium transporter (TRK1) was cloned by its ability to relieve the potassium transport defect in trk1 cells. TRK1 encodes a protein 1,235 amino acids long that contains 12 potential membrane-spanning domains. Our results demonstrate the physical and functional independence of the yeast potassium and proton transport systems. TRK1 is nonessential in S. cerevisiae and maps to a locus unlinked to PMA1, the gene that encodes the plasma membrane ATPase. Haploid cells that contain a null allele of TRK1 (trk1.DELTA.) rely on a low-affinity transporter for potassium uptake and, under certain conditions, exhibit energy-dependent loss of potassium, directly exposing the activity of a transporter resonsible for the efflux of this ion.