Membrane fusion: Stalk model revisited
Membrane fusion: Stalk model revisited
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DOI：
10.1016/s0006-3495(02)75432-5
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发表时间：
2002-02-01
影响因子：
3.4
通讯作者：
Albanesi, JP
中科院分区：
文献类型：
--
作者：
Markin, VS;Albanesi, JP
膜融合被认为是通过称为茎的中间结构进行的。对秸秆的数学分析提供了这一结构所涉及的弹性能量，并预测了整个过程可能的演变，但原始模型预测的能量高得令人怀疑。这是由于一个错误的假设，即茎具有圆弧的旋转图形。这里我们放弃这个假设，计算出茎的正确形状。我们发现，它可以完全无应力，因此，它的能量可以变成负的，而不是正的和高的，从而促进了聚变过程。在新计算的基础上，分析了半熔融能、完全熔融能和双层膜中孔道的能量。对膜融合和脂质相变的意义进行了讨论。
Membrane fusion is believed to proceed via intermediate structures called stalks. Mathematical analysis of the stalk provided the elastic energy involved in this structure and predicted the possible evolution of the overall process, but the energies predicted by the original model were suspiciously high. This was due to an erroneous assumption, i.e., that the stalk has a figure of revolution of a circular arc. Here we abandon this assumption and calculate the correct shape of the stalk. We find that it can be made completely stress free and, hence, its energy, instead of being positive and high can become negative, thus facilitating the fusion process. Based on our new calculations, the energies of hemifusion, of complete fusion, and of the pore in a bilayer were analyzed. Implications for membrane fusion and lipid phase transitions are discussed.