Genetic transfer of nitrogenase–hydrogenase activity in Rhodopseudomonas capsulata
Genetic transfer of nitrogenase–hydrogenase activity in Rhodopseudomonas capsulata
复制标题
荚膜红假单胞菌固氮酶-氢化酶活性的遗传转移
作者：
J. Wall;P. Weaver;H. Gest
1971年，在肺炎克雷伯菌中首次观察到固氮（nif）基因通过转导和接合2转移到这种能力缺陷的细菌突变体（nif -）。所引用的研究以及随后证明nif基因可以从肺炎克雷伯菌转移到大肠杆菌的研究被广泛认为是固氮过程分子生物学的先兆。我们现在报告了在这方面感兴趣的一个新系统，即nif基因在无硫紫色光合细菌Rhodopseudomonas capsulata中的转移。这种生物的遗传转移是由一种小体积、性质未知的可过滤载体介导的，即Marrs4发现的“基因转移剂”（GTA）。
THE transfer of nitrogen fixation (nif) genes to bacterial mutants defective in this capacity (nif−) by transduction1 and conjugation2 was first observed in 1971 with Klebsiella pneumoniae. The investigations cited and the subsequent demonstration3 that nif genes can be transferred from K. pneumoniae to Escherichia coli have been widely regarded as harbingers of the molecular biology of the nitrogen fixation process. We now report a new system of interest in this connection, namely, the transfer of nif genes in the non-sulphur purple photosynthetic bacterium Rhodopseudomonas capsulata. Genetic transfer in this organism is mediated by a filterable vector of small size and unknown nature, the “gene transfer agent” (GTA), discovered by Marrs4.