Magnesium Oxide nanoparticle effect on the growth, development, and microRNAs expression of Ananas comosus var. bracteatus
Magnesium Oxide nanoparticle effect on the growth, development, and microRNAs expression of Ananas comosus var. bracteatus
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DOI：
10.1080/17429145.2021.1931720
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发表时间：
2021-01
影响因子：
3.2
通讯作者：
Mark Owusu Adjei;Xuzixing Zhou;Meiqin Mao;Yanbin Xue;Jiawen Liu;Hao Hu;Jiaheng Luo;Huiling Zhang
中科院分区：
文献类型：
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作者：
Mark Owusu Adjei;Xuzixing Zhou;Meiqin Mao;Yanbin Xue;Jiawen Liu;Hao Hu;Jiaheng Luo;Huiling Zhang
摘要使用适当比例的纳米颗粒来促进植物生长和发育是一个正在进行的生物过程。然而，其暴露在一个微不足道的比例成为压力和植物使用不同的调节机制，如基因表达和miRNA的调节，以达到其细胞内稳态。本研究记录了MgO纳米颗粒对菠萝基因和miRNA表达、细胞形态、叶绿素含量和生理变化的影响。在不同处理水平下的小苞片。研究发现，随着MgO纳米颗粒暴露于植物的增加，平均根长、平均生物量和平均叶长显著降低。低浓度的纳米氧化镁（1 g/mL）可提高叶片叶绿素含量，而2 g/mL和4 g/mL则显著降低叶片叶绿素含量。4 μ g/mL的MgO纳米颗粒显著增加了miR-396和miR-398的表达，同时显著抑制了RHS 12和XTH基因的表达。4g/mL纳米氧化镁处理明显减少了根中柱的维管束数量。20 nm MgO纳米颗粒可能对菠萝的生长发育产生破坏性影响。bracteatus通过抑制叶绿素。
ABSTRACT The use of nanoparticles in the appropriate portion to increase plant growth and development is an ongoing biological process. However, its exposure in an inconsiderable proportion become stressful and plant uses diverse regulatory mechanisms such as gene expression and miRNAs regulation to attain their cellular homeostasis. This study documented the influence of MgO NPs on genes and miRNAs expression, cell morphology, chlorophyll content, and physiological changes in Ananas comosus var. bracteatus at different treatment levels. It’s found that as MgO NPs exposed to the plant's increased, the average root length, average biomass, and average leaf length significantly decreased. A low concentration of MgO NPs (1 g/mL) increased the chlorophyll content of leaves, while 2 and 4 g/mL decreased it significantly. A 4 g/mL of MgO NPs increased the expression of miR-396 and miR-398 extremely, while repressed the expression of RHS12 and XTH genes significantly. Furthermore, the number of vascular bundles of root stele decreased obviously under 4 g/mL MgO NPs. 20 nm MgO NPs may have a destructive influence on the growth and development of Ananas comosus var. bracteatus by inhibiting chlorophyll.