ACETYLCHOLINESTERASE ACTIVITY AND TYPE-C SYNAPSES IN THE HYPOGLOSSAL, FACIAL AND SPINAL-CORD MOTOR NUCLEI OF RATS - AN ELECTRON-MICROSCOPE STUDY
ACETYLCHOLINESTERASE ACTIVITY AND TYPE-C SYNAPSES IN THE HYPOGLOSSAL, FACIAL AND SPINAL-CORD MOTOR NUCLEI OF RATS - AN ELECTRON-MICROSCOPE STUDY
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DOI：
10.1007/bf00482985
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发表时间：
1986-01-01
期刊：
影响因子：
--
通讯作者：
IRINTCHEV, AP
中科院分区：
文献类型：
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作者：
DAVIDOFF, MS;IRINTCHEV, AP
利用 Lewis 和 Shute 的电子显微镜技术，我们研究了大鼠舌下、面部和脊髓运动核中乙酰胆碱酯酶 (AChE) 活性的定位。所使用的技术选择性地检测具有突触下池的突触（C型突触）以及粗面内质网、核膜碎片、一些高尔基体区以及部分周质膜、轴突和树突膜上反应产物的大量沉积。在 C 突触中，AChE 活性位于突触间隙和突触前纽扣膜上。一些 C 突触以多个“活动区”的形式表现出明显的突触专门化。这些区域的特征是密集的突触前投射、突触间隙的短暂扩张以及位于突触后膜和外膜以及突触下池外膜之间的突触后密度。在突触后密度内，观察到成排的杆状或通道状结构。突触下池与阳性粗面内质网连续。讨论了 C 突触在调节突触后胆碱能神经元 AChE 合成和/或调节 AChE 释放到细胞外空间以及建立新突触接触中的可能作用。
Using the electron-microscope technique of Lewis and Shute, we studied the localization of the acetylcholinesterase (AChE) activity in the hypoglossal, facial and spinal-cord motor nuclei of rats. The technique used selectively detects synapses with subsynaptic cisterns (type C synapses) as well as heavy deposits of reaction products in the rough endoplasmic reticulum, in fragments of the nuclear envelope, in some Golgi zones and on parts of the pericaryal plasma membrane, the axolemma and the dendritic membrane. In C synapses, AChE activity was located in the synaptic cleft and on the membrane of presynaptic boutons. Some C synapses exhibited distinct synaptic specialization in the form of multiple ''active zones''. These zones were characterized by dense presynaptic projections, short dilations of the synaptic cleft, and postsynaptic densities localized between the postsynaptic membrane and the outer membrane and the outer membrane of the subsynaptic cistern. Within the postsynaptic densities, rows of rod- or channel-like structures were observed. The subsynaptic cisterns were continuous with the positive rough endoplasmic reticulum. The results are discussed in terms of the possible role of C synapses in the regulation of AChE synthesis in postsynaptic cholinergic neurons and/or in the regulation of AChE release into the extracellular space as well as in the establishment of new synaptic contacts.