Canonical eukaryotic initiation factors determine initiation of translation by internal ribosomal entry
Canonical eukaryotic initiation factors determine initiation of translation by internal ribosomal entry
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DOI：
10.1128/mcb.16.12.6859
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发表时间：
1996-12-01
影响因子：
5.3
通讯作者：
Shatsky, IN
中科院分区：
文献类型：
--
作者：
Pestova, TV;Hellen, CUT;Shatsky, IN
小核糖核酸病毒RNA的翻译在核糖体结合到5'非翻译区内的内部核糖体进入位点（IRES）后开始。我们已经重建IRES介导的启动脑心肌炎病毒RNA纯化的成分，并使用引物延伸分析，以确认48 S preinitiation复合物形成的保真度。真核起始因子2（eIF 2），eIF 3，和eIF 4F所需的启动; eIF 4 B和在较小程度上的嘧啶trac-binding蛋白刺激这一过程。我们表明，eIF 4F结合到IRES在一个新的帽独立的方式，并建议帽和IRES依赖的启动机制利用不同的模式与此因子的相互作用，以促进核糖体附着到mRNA。
Translation of picornavirus RNA is initiated after ribosomal binding to an internal ribosomal entry site (IRES) within the 5' untranslated region. We have reconstituted IRES-mediated initiation on encephalomyocarditis virus RNA from purified components and used primer extension analysis to confirm the fidelity of 48S preinitiation complex formation. Eukaryotic initiation factor 2 (eIF2), eIF3, and eIF4F were required for initiation; eIF4B and to a lesser extent the pyrimidine tract-binding protein stimulated this process. We show that eIF4F binds to the IRES in a novel cap-independent manner and suggest that cap- and IRES-dependent initiation mechanisms utilize different modes of interaction with this factor to promote ribosomal attachment to mRNA.