An analysis of the components in UW solution using the isolated perfused rabbit liver.
An analysis of the components in UW solution using the isolated perfused rabbit liver.
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使用离体灌注兔肝脏对 UW 溶液中的成分进行分析。
DOI：
10.1097/00007890-198810000-00009
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发表时间：
1988
期刊：
影响因子：
6.2
通讯作者：
Belzer,FO
中科院分区：
文献类型：
--
作者：
Jamieson,NV;Lindell,S;Sundberg,R;Southard,JH;Belzer,FO
用离体灌注兔肝模型测定单纯冷藏保肝用UW液的主要成分。用待测溶液初始冲洗后，肝脏在冰上保存48小时，然后在38[度]C的隔离灌注回路中重新灌注；记录两小时内的胆汁流量和酶（SGOT、SGPT和LDH）释放。所有测试的溶液都含有磷酸盐（25毫米）作为缓冲液和硫酸镁（5毫米）。钠可以代替钾而没有副作用。乳糖酸盐、棉子糖和谷胱甘肽不能省略；在此模型中，所有其他组件都可以在不改变解决方案有效性的情况下消除。
The isolated perfused rabbit liver model has been used to determine the essential components of the UW solution for hepatic preservation by simple cold storage. Livers were stored on ice for 48 hr after initial flushing with the solution being tested, and then reperfused at 38 [degrees] C in an isolated perfusion circuit; bile flow and enzyme (SGOT, SGPT, and LDH) release during a 2-hr period were recorded. All solutions tested contained phosphate (25 mM) as a buffer and magnesium sulfate (5 mM). Sodium can be substituted for potassium without adverse effects. Lactobionate, raffinose and glutathione cannot be omitted; all other components can be eliminated without altering the effectiveness of the solution in this model.