Lateral reorganization of plasma membrane is involved in the yeast resistance to severe dehydration
Lateral reorganization of plasma membrane is involved in the yeast resistance to severe dehydration
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DOI：
10.1016/j.bbamem.2010.01.015
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发表时间：
2010-05-01
影响因子：
3.4
通讯作者：
Gervais, Patrick
中科院分区：
文献类型：
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作者：
Dupont, Sebastien;Beney, Laurent;Gervais, Patrick
在这项研究中，我们研究了酵母质膜（PM）修饰的动力学和脱水程度，因为这一参数对细胞存活至关重要。在进行性（非致死性）和快速（致死性）高渗扰动下，通过共聚焦和电子显微镜研究了PM修饰的功能（渗透性）和结构（富含甾醇的膜微域中含有的蛋白质Sur7-GFP的形态、超微结构和分布）。快速的细胞脱水诱导了许多PM内陷的形成，随着时间的推移，低固醇含量PM区域的膜内化。由于细胞膜表面的缺陷，在再水化阶段质膜发生渗透，导致细胞死亡。逐渐脱水导致一些大的PM褶皱的形成而没有膜内化。这也导致了Sur7-GFP微结构域分布的改变，表明发生了膜组分的横向重排。这一事件是时间的函数，并涉及PM在渐进扰动期间的特定变形。微畴在快速扰动期间的重新划分巩固了这一假设。这些发现强调了扰动动力学影响PM组织的进化，并表明PM横向重组在细胞存活中对水扰动的关键作用。(C) 2010 Elsevier B.V.版权所有
In this study, we investigated the kinetic and the magnitude of dehydrations on yeast plasma membrane (PM) modifications because this parameter is crucial to cell survival. Functional (permeability) and structural (morphology, ultrastructure, and distribution of the protein Sur7-GFP contained in sterol-rich membrane microdomains) PM modifications were investigated by confocal and electron microscopy after progressive (non-lethal) and rapid (lethal) hyperosmotic perturbations. Rapid cell dehydration induced the formation of many PM invaginations followed by membrane internalization of low sterol content PM regions with time. Permeabilization of the plasma membrane occurred during the rehydration stage because of inadequacies in the membrane surface and led to cell death. Progressive dehydration conducted to the formation of some big PM pleats without membrane internalization. It also led to the modification of the distribution of the Sur7-GFP microdomains, suggesting that a lateral rearrangement of membrane components occurred. This event is a function of time and is involved in the particular deformations of the PM during a progressive perturbation. The maintenance of the repartition of the microdomains during rapid perturbations consolidates this assumption. These findings highlight that the perturbation kinetic influences the evolution of the PM organization and indicate the crucial role of PM lateral reorganization in cell survival to hydric perturbations. (C) 2010 Elsevier B.V. All rights reserved.