Controlled aggregation of superparamagnetic iron oxide nanoparticles for the development of molecular magnetic resonance imaging probes
Controlled aggregation of superparamagnetic iron oxide nanoparticles for the development of molecular magnetic resonance imaging probes
复制标题
DOI：
10.1088/0957-4484/19/26/265102
复制
发表时间：
2008-07-02
期刊：
影响因子：
3.5
通讯作者：
Stoldt, C. R.
中科院分区：
文献类型：
--
作者：
Larsen, B. A.;Haag, M. A.;Stoldt, C. R.
描述了一种用于合成作为分子磁共振成像（MRI）造影剂的超顺磁性氧化铁（SPIO）多纳米颗粒聚集体的方法。该方法利用胶体中的有机酸/碱相互作用来诱导高度可控的纳米颗粒聚集。通过控制SPIO纳米粒子在四氢呋喃溶剂中的界面化学，合成了直径达100 nm的单分散聚集体。随后的磷脂胶束包封产生胶束多SPIO（mmSPIO）聚集体，与胶束单颗粒SPIO（302.0 s（-1）mmol（-1）Fe）相比，其具有增强的T（2）弛豫率（368.0 s（-1）mmol（-1）Fe）。将与抗CA 125单克隆抗体偶联的mmSPIO与卵巢癌细胞系一起孵育，以证明体外靶向分子MRI，导致CA 125阳性NIH：OVCAR-3细胞的T（2）时间缩短66%，CA 125阴性SK-OV-3细胞的T（2）时间变化小于3%。mmSPIO的可控聚集显示出开发具有用于特定诊断成像应用的最佳尺寸的分子MRI造影剂的潜力。
A method for synthesizing superparamagnetic iron oxide ( SPIO) multi-nanoparticle aggregates as molecular magnetic resonance imaging (MRI) contrast agents is described. The approach utilizes organic acid/base interactions in the colloid to induce highly controllable nanoparticle aggregation. Monodisperse aggregates with diameters as large as 100 nm are synthesized by manipulating the interfacial surface chemistry of the SPIO nanoparticles in tetrahydrofuran solvent. Subsequent phospholipid micelle encapsulation yields micellar multi-SPIO (mmSPIO) aggregates with enhanced T(2) relaxivity (368.0 s(-1) mmol(-1) Fe) as compared to micellar single particle SPIO (302.0 s(-1) mmol(-1) Fe). mmSPIO conjugated to anti-CA125 monoclonal antibodies were incubated with ovarian carcinoma cell lines to demonstrate targeted in vitro molecular MRI, resulting in a 66% shortening in T(2) time for CA125 positive NIH: OVCAR-3 cells and a less than 3% change in T(2) time for CA125 negative SK-OV-3 cells. The controllable aggregation of mmSPIO shows potential for the development of molecular MRI contrast agents with optimal sizes for specific diagnostic imaging applications.