Blood-Brain Barrier Opening in Behaving Non-Human Primates via Focused Ultrasound with Systemically Administered Microbubbles.
Blood-Brain Barrier Opening in Behaving Non-Human Primates via Focused Ultrasound with Systemically Administered Microbubbles.
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DOI：
10.1038/srep15076
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发表时间：
2015-10-26
影响因子：
4.6
通讯作者：
Ferrera VP
中科院分区：
文献类型：
--
作者：
Downs ME;Buch A;Karakatsani ME;Konofagou EE;Ferrera VP
在过去的十五年中，聚焦超声结合静脉注射微泡（FUS）已被证明是一种有效的，非侵入性的技术，在体内打开血脑屏障（BBB）。在这里，我们表明，FUS可以安全有效地打开BBB在基底神经节和丘脑在警觉的非人灵长类动物（NHP），而他们执行的行为任务。在89%的病例中，在警戒NHP的靶脑区域成功打开了BBB，平均打开体积比之前的麻醉FUS手术大28%。与麻醉手术相比，FUS在警戒期间的安全性（无水肿或微出血）也有所改善。在任何手术过程中均未观察到生理效应（心率变化、运动诱发电位）。此外，FUS的应用并没有破坏达到的行为，但实际上通过减少反应时间23毫秒，并显着减少触摸误差平均0.76毫米，提高了性能。
Over the past fifteen years, focused ultrasound coupled with intravenously administered microbubbles (FUS) has been proven an effective, non-invasive technique to open the blood-brain barrier (BBB) in vivo. Here we show that FUS can safely and effectively open the BBB at the basal ganglia and thalamus in alert non-human primates (NHP) while they perform a behavioral task. The BBB was successfully opened in 89% of cases at the targeted brain regions of alert NHP with an average volume of opening 28% larger than prior anesthetized FUS procedures. Safety (lack of edema or microhemorrhage) of FUS was also improved during alert compared to anesthetized procedures. No physiological effects (change in heart rate, motor evoked potentials) were observed during any of the procedures. Furthermore, the application of FUS did not disrupt reaching behavior, but in fact improved performance by decreasing reaction times by 23 ms, and significantly decreasing touch error by 0.76 mm on average.