Riemerella anatipestifer outbreaks in commercial goose flocks and identification of isolates by MALDI-TOF mass spectrometry
Riemerella anatipestifer outbreaks in commercial goose flocks and identification of isolates by MALDI-TOF mass spectrometry
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DOI：
10.1080/03079457.2013.775401
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发表时间：
2013-04-01
期刊：
影响因子：
2.8
通讯作者：
Hess, M.
中科院分区：
文献类型：
--
作者：
Hess, C.;Enichlmayr, H.;Hess, M.
在很短的时间内，在商品鹅中发生了几起鸭疫里默氏杆菌暴发。一种严重的疾病是公认的受影响的鸟类，主要特点是抑郁症和严重的神经功能障碍。发病率为20 ~ 30%，死亡率为5 ~ 20%。一般来说，临床症状开始于8至10天的年龄。剖检结果显示所有禽鸟均出现病毒性心包炎、肝周炎及气囊炎。有些鸡的胫跗关节也有滑膜炎，这些关节和跖骨周围的皮下组织水肿肿胀。组织学检查显示不同器官中存在特征性严重炎症，伴嗜异性粒细胞。对几种器官进行了细菌学调查，并对R.从所有调查的鸟类中均能分离到鸭疫病毒。首次通过基质辅助激光解吸电离飞行时间质谱法对这些临床分离株进行鉴定，证实了病原学。序列分析显示，临床分离株之间的相似性为100%，表明共同的感染源。
Several outbreaks of Riemerella anatipestifer in commercial geese occurred within a short time period. A serious disease was recognized in the affected birds, mainly characterized by depression and severe neurologic disturbances. The morbidity ranged from 20 to 30% and the mortality from 5 to 20%. Generally, the clinical signs started at the age of 8 to 10 days. Post-mortem examination revealed fibrinous pericarditis, perihepatitis and airsacculitis in all birds. Some of the birds also had synovitis of the tibio-tarsal joints and oedematous swelling of the subcutaneous tissues around these joints and metatarsus. Histology revealed a characteristic severe inflammation with heterophilic granulocytes in different organs. Bacteriological investigation was made from several organs and R. anatipestifer could be isolated from all birds investigated. The identification of these clinical isolates, done for the first time by matrix-assisted laser desorption ionizationtime-of-flight mass spectrometry, confirmed the aetiology. Sequence analysis showed 100% similarity between the clinical isolates, indicating a common source of infection.